TNIK Antibody - #DF9050

(1)  
Product: TNIK Antibody
Catalog: DF9050
Description: Rabbit polyclonal antibody to TNIK
Application: WB
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 155 kDa; 155kD(Calculated).
Uniprot: Q9UKE5
RRID: AB_2842246

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TNIK Antibody detects endogenous levels of total TNIK.
RRID:
AB_2842246
Cite Format: Affinity Biosciences Cat# DF9050, RRID:AB_2842246.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.11.1; KIAA0551; Tnik; TNIK_HUMAN; Traf2 and NCK interacting kinase; Traf2 and NCK-interacting protein kinase;

Immunogens

Immunogen:
Uniprot:

Q9UKE5(TNIK_HUMAN) >>Visit HPA database.

Gene(ID):
TNIK(23043)
Expression:
Q9UKE5 TNIK_HUMAN:

Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.

Sequence:
MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRPVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPILESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPAKVKPEESRDITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPMKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGTEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Pig
67
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKE5 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
Y36 Phosphorylation
Y40 Phosphorylation
K46 Ubiquitination
Y86 Phosphorylation
K121 Ubiquitination
K155 Ubiquitination
S175 Phosphorylation
T181 Phosphorylation
T187 Phosphorylation
K303 Ubiquitination
T319 Phosphorylation
Y321 Phosphorylation
Y323 Phosphorylation
S324 Phosphorylation
S326 Phosphorylation
S335 Phosphorylation
Y427 Phosphorylation
Y446 Phosphorylation
Y475 Phosphorylation
Y499 Phosphorylation
S502 Phosphorylation
Y519 Phosphorylation
Y521 Phosphorylation
S526 Phosphorylation
S541 Phosphorylation
S548 Phosphorylation
S560 Phosphorylation
S568 Phosphorylation
S570 Phosphorylation
S574 Phosphorylation
T581 Phosphorylation
S600 Phosphorylation
S608 Phosphorylation
S610 Phosphorylation
S640 Phosphorylation
T643 Phosphorylation
S660 Phosphorylation
T677 Phosphorylation
S678 Phosphorylation
S680 Phosphorylation
S688 Phosphorylation
R698 Methylation
S701 Phosphorylation
R705 Methylation
S707 Phosphorylation
S720 Phosphorylation
S726 Phosphorylation
S729 Phosphorylation
S755 Phosphorylation
S764 Phosphorylation
S766 Phosphorylation
S769 Phosphorylation
S795 Phosphorylation
K824 Acetylation
S892 Phosphorylation
T902 Phosphorylation
S938 Phosphorylation
T942 Phosphorylation
S954 Phosphorylation
S959 Phosphorylation
Y963 Phosphorylation
S972 Phosphorylation
Y982 Phosphorylation
T987 Phosphorylation
S1021 Phosphorylation
T1036 Phosphorylation
S1116 Phosphorylation
K1131 Ubiquitination
K1178 Ubiquitination
Y1264 Phosphorylation
T1267 Phosphorylation
K1319 Ubiquitination
K1325 Ubiquitination
K1333 Ubiquitination

PTMs - Q9UKE5 As Enzyme

Substrate Site Source
Q15797 (SMAD1) T322 Uniprot
Q9NQB0 (TCF7L2) S177 Uniprot
Q9UKE5-1 (TNIK) T181 Uniprot
Q9UKE5-1 (TNIK) S640 Uniprot
Q9UKE5-1 (TNIK) S680 Uniprot

Research Backgrounds

Function:

Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.

PTMs:

Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.

Subcellular Location:

Nucleus. Cytoplasm. Recycling endosome. Cytoplasm>Cytoskeleton.
Note: Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.

Subunit Structure:

Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.