CCNB2 Antibody - #DF9047

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Product: CCNB2 Antibody
Catalog: DF9047
Description: Rabbit polyclonal antibody to CCNB2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 45 kDa; 45kD(Calculated).
Uniprot: O95067
RRID: AB_2842243

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
CCNB2 Antibody detects endogenous levels of total CCNB2.
RRID:
AB_2842243
Cite Format: Affinity Biosciences Cat# DF9047, RRID:AB_2842243.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ccnb2; CCNB2_HUMAN; CycB2; Cyclin B2; G2 mitotic specific cyclin B2; G2/mitotic specific cyclin B2; G2/mitotic-specific cyclin-B2; HsT17299; MGC108931; MGC140694;

Immunogens

Immunogen:
Uniprot:

O95067(CCNB2_HUMAN) >>Visit HPA database.

Gene(ID):
CCNB2(9133)
Sequence:
MALLRRPTVSSDLENIDTGVNSKVKSHVTIRRTVLEEIGNRVTTRAAQVAKKAQNTKVPVQPTKTTNVNKQLKPTASVKPVQMEKLAPKGPSPTPEDVSMKEENLCQAFSDALLCKIEDIDNEDWENPQLCSDYVKDIYQYLRQLEVLQSINPHFLDGRDINGRMRAILVDWLVQVHSKFRLLQETLYMCVGIMDRFLQVQPVSRKKLQLVGITALLLASKYEEMFSPNIEDFVYITDNAYTSSQIREMETLILKELKFELGRPLPLHFLRRASKAGEVDVEQHTLAKYLMELTLIDYDMVHYHPSKVAAAASCLSQKVLGQGKWNLKQQYYTGYTENEVLEVMQHMAKNVVKVNENLTKFIAIKNKYASSKLLKISMIPQLNSKAVKDLASPLIGRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
92
Chicken
75
Zebrafish
67
Xenopus
58
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95067 As Substrate

Site PTM Type Enzyme
T8 Phosphorylation
S10 Phosphorylation
S11 Phosphorylation
T18 Phosphorylation
S22 Phosphorylation
K23 Ubiquitination
K25 Ubiquitination
K57 Ubiquitination
K64 Ubiquitination
K73 Ubiquitination
S77 Phosphorylation
K79 Ubiquitination
K85 Ubiquitination
S92 Phosphorylation
T94 Phosphorylation
S99 Phosphorylation
K101 Ubiquitination
S204 Phosphorylation
S220 Phosphorylation
K258 Ubiquitination
S274 Phosphorylation
K275 Ubiquitination
Y298 Phosphorylation
K318 Ubiquitination
K324 Ubiquitination
K328 Ubiquitination
K349 Ubiquitination
K353 Ubiquitination
T359 Phosphorylation
K360 Ubiquitination
K367 Ubiquitination
Y368 Phosphorylation
K372 Ubiquitination
K375 Ubiquitination
K385 Ubiquitination
K388 Ubiquitination
S392 Phosphorylation
S398 Phosphorylation

Research Backgrounds

Function:

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Subunit Structure:

Interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.

Family&Domains:

Belongs to the cyclin family. Cyclin AB subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

 

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