Phospho-AhR (Ser36) Antibody | Affinity Biosciences

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AF3278 at 1/100 staining human Brain tissue sections by IHC-P.

Figure 8 FOR increases AhR expression and inhibits the phosphorylation level of Src.

Product:	Phospho-AhR (Ser36) Antibody
Catalog:	AF3278
Description:	Rabbit polyclonal antibody to Phospho-AhR (Ser36)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	90kDa; 96kD(Calculated).
Uniprot:	P35869
RRID:	AB_2834506

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Data Sheet

COA

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-AhR (Ser36) Antibody detects endogenous levels of AhR only when phosphorylated at Serine 36.

RRID:

AB_2834506
Cite Format: Affinity Biosciences Cat# AF3278, RRID:AB_2834506.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ah receptor; AhR; AHR_HUMAN; Aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; Aryl hydrocarbon receptor precursor; bHLHe76; Class E basic helix loop helix protein 76; Class E basic helix-loop-helix protein 76; HGNC:348;

Immunogens

Immunogen:

Uniprot:

P35869(AHR_HUMAN) >>Visit HPA database.

Gene(ID):

AHR(196) | AHRR(57491)

Expression:

P35869 AHR_HUMAN:

Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. Expressed in retinal photoreceptors (PubMed:29726989).

Description:

The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Sequence:

P35869 AHR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGSGYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLTDEEGTEHLRKRNTKLPFMFTTGEAVLYEATNPFPAIMDPLPLRTKNGTSGKDSATTSTLSKDSLNPSSLLAAMMQQDESIYLYPASSTSSTAPFENNFFNESMNECRNWQDNTAPMGNDTILKHEQIDQPQDVNSFAGGHPGLFQDSKNSDLYSIMKNLGIDFEDIRHMQNEKFFRNDFSGEVDFRDIDLTDEILTYVQDSLSKSPFIPSDYQQQQSLALNSSCMVQEHLHLEQQQQHHQKQVVVEPQQQLCQKMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYPMGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Horse

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35869 As Substrate

P35869

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S12	Phosphorylation	P17252 (PRKCA)	Uniprot
K24	Acetylation		Uniprot
K24	Sumoylation		Uniprot
K24	Ubiquitination		Uniprot
K32	Ubiquitination		Uniprot
S36	Phosphorylation	P17252 (PRKCA)	Uniprot
T45	Phosphorylation		Uniprot
K63	Ubiquitination		Uniprot
S68	Phosphorylation		Uniprot
S75	Phosphorylation		Uniprot
Y76	Phosphorylation		Uniprot
S81	Phosphorylation		Uniprot
K88	Ubiquitination		Uniprot
S232	Phosphorylation		Uniprot
K244	Ubiquitination		Uniprot
K254	Sumoylation		Uniprot
K292	Ubiquitination		Uniprot
Y322	Phosphorylation		Uniprot
T355	Phosphorylation		Uniprot
Y378	Phosphorylation		Uniprot
K438	Ubiquitination		Uniprot
K510	Ubiquitination		Uniprot
K535	Ubiquitination		Uniprot
Y540	Phosphorylation		Uniprot
K544	Ubiquitination		Uniprot
K560	Ubiquitination		Uniprot
K641	Ubiquitination		Uniprot

Research Backgrounds

P35869_AHR_HUMAN

Function:

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.

PTMs:

Mono-ADP-ribosylated, leading to inhibit transcription activator activity of AHR.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.

Tissue Specificity:

Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. Expressed in retinal photoreceptors.

Subunit Structure:

Homodimer (By similarity). Binds MYBBP1A (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. Interacts with NEDD8 and IVNS1ABP. Interacts with ARNTL/BMAL1. Interacts with HSP90AB1 (By similarity). Interacts with ARNT; the heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription. Interacts with TIPARP; leading to mono-ADP-ribosylation of AHR and subsequent inhibition of AHR.

Family&Domains:

The PAS 1 domain is essential for dimerization and also required for AHR:ARNT heterodimerization.

Research Fields

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

References

1). Formononetin Protects LPS-Induced Mastitis Through Suppressing Inflammation and Enhancing Blood-Milk Barrier Integrity via AhR-Induced Src Inactivation. Frontiers in Immunology, 2022 (PubMed: 35185907) [IF=7.3]

Application: WB Species: Mice Sample: EpH4-Evs cells

Figure 8 FOR increases AhR expression and inhibits the phosphorylation level of Src. The present results are displayed as the mean ± SEM of triple parallel measurements. #(P < 0.01) is significantly different from control group; **(P < 0.01) is significantly different from LPS group.

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Phospho-AhR (Ser36) Antibody - #AF3278