WNT4 Antibody | Affinity Biosciences

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FIGURE 6 FKN deficiency suppressed macrophages polarization and proliferation in mice kidney tissues via inhibition of Wnt/β-catenin signalling.

FIGURE 2|FKN promoted the viability of J774A.

Product:	WNT4 Antibody
Catalog:	DF9040
Description:	Rabbit polyclonal antibody to WNT4
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	39 kDa; 39kD(Calculated).
Uniprot:	P56705
RRID:	AB_2842236

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

WNT4 Antibody detects endogenous levels of total WNT4.

RRID:

AB_2842236
Cite Format: Affinity Biosciences Cat# DF9040, RRID:AB_2842236.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC123964; Protein Wnt-4; RP23-246F18.1; SERKAL; Wingless type MMTV integration site family member 4 precursor; WNT 4; WNT 4 protein precursor; WNT4; WNT4_HUMAN;

Immunogens

Immunogen:

Uniprot:

P56705(WNT4_HUMAN) >>Visit HPA database.

Gene(ID):

WNT4(54361)

Sequence:

P56705 WNT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSPRSCLRSLRLLVFAVFSAAASNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDMRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCSCKFHWCCFVKCRQCQRLVELHTCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56705 As Substrate

P56705

Site	PTM Type	Source
S66	Phosphorylation	Uniprot
T106	Phosphorylation	Uniprot
Y113	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
K196	Acetylation	Uniprot
T295	Phosphorylation	Uniprot

Research Backgrounds

P56705_WNT4_HUMAN

Function:

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung. Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Required for normal lung development and for normal patterning of trachael cartilage rings (By similarity).

PTMs:

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Subunit Structure:

Interacts with PORCN (By similarity). Interacts with PKD1.

Family&Domains:

Belongs to the Wnt family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

References

1). Fractalkine Aggravates LPS-induced Macrophage Activation and Acute Kidney Injury via Wnt/β-catenin Signaling Pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 34101346) [IF=5.3]

Application: WB Species: Mice Sample: kidney tissues

FIGURE 6 FKN deficiency suppressed macrophages polarization and proliferation in mice kidney tissues via inhibition of Wnt/β-catenin signalling. A, B, Western blot analysis and their respective quantitation showing the protein expression of FKN, β-catenin, Wnt-4, c-myc, cyclinD1, iNOS, TNF-α, ARG-1 and IL-10 in mice kidney tissues. *P < .05 compared with the WT mice; #P < .05 compared with the LPS mice

Application: WB Species: mouse Sample: J774A. 1 cells

FIGURE 2|FKN promoted the viability of J774A.1 cells via Wnt/β-catenin signalling.D, E,Western blotting analysis and their respective quantitation showing the protein expression of FKN, β-catenin,Wnt-4, c-myc and cyclinD1 in J774A.1 cells.

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WNT4 Antibody - #DF9040