Product: CYB5R4 Antibody
Catalog: DF9025
Description: Rabbit polyclonal antibody to CYB5R4
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 59 kDa; 59kD(Calculated).
Uniprot: Q7L1T6
RRID: AB_2842221

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
CYB5R4 Antibody detects endogenous levels of total CYB5R4.
RRID:
AB_2842221
Cite Format: Affinity Biosciences Cat# DF9025, RRID:AB_2842221.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

b5+b5R; b5b5r; C79736; cb5/cb5R; Cb5cb5r; Cyb5r4; Cytochrome b type NAD(P)H oxidoreductase; Cytochrome b5 reductase 4; dJ676J13.1; Flavohemoprotein b5+b5R; Flavohemoprotein b5/b5R; N terminal cytochrome b5 and cytochrome b5 oxidoreductase domain containing protein; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; NADPH cytochrome B5 oxidoreductase; NB5R4_HUMAN; NCB5OR; RP4 676J13.1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q7L1T6 NB5R4_HUMAN:

Widely expressed.

Sequence:
MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESMLKECLVGRMAIKPAVLKDYREEEKKVLNGMLPKSQVTDTLAKEGPSYPSYDWFQTDSLVTIAIYTKQKDINLDSIIVDHQNDSFRAETIIKDCLYLIHIGLSHEVQEDFSVRVVESVGKIEIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQLISKEDVTHDTRLFCLMLPPSTHLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYFLIKIYPTGLFTPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLRKVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLVCICGPVPFTEQGVRLLHDLNFSKNEIHSFTA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Pig
92
Xenopus
92
Zebrafish
75
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L1T6 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S14 Phosphorylation
S154 Phosphorylation
T157 Phosphorylation
K277 Ubiquitination
Y326 Phosphorylation
T361 Phosphorylation
K442 Ubiquitination
S471 Phosphorylation
S476 Phosphorylation

Research Backgrounds

Function:

NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin.

Subcellular Location:

Endoplasmic reticulum.
Note: Soluble protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Family&Domains:

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

Restrictive clause

 

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For Research Use Only.
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