MYST3 Antibody - #DF9024

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Product: MYST3 Antibody
Catalog: DF9024
Description: Rabbit polyclonal antibody to MYST3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 225 kDa; 225kD(Calculated).
Uniprot: Q92794
RRID: AB_2842220

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
MYST3 Antibody detects endogenous levels of total MYST3.
RRID:
AB_2842220
Cite Format: Affinity Biosciences Cat# DF9024, RRID:AB_2842220.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HAT 3; Histone acetyltransferase MYST3; Monocytic leukemia zinc finger protein; MOZ; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3; MYST histone acetyltransferase (monocytic leukemia) 3; MYST protein 3; MYST-3; MYST3; MYST3_HUMAN; OTTHUMP00000197392; OTTHUMP00000197394; OTTHUMP00000225722; Runt related transcription factor binding protein 2; Runt-related transcription factor-binding protein 2; RUNXBP2; SAS2; SAS2 and TIP60 protein 3; TIP60 protein 3; YBF2/SAS3; ZC2HC6A; Zinc finger protein 220; ZNF220;

Immunogens

Immunogen:
Uniprot:

Q92794(KAT6A_HUMAN) >>Visit HPA database.

Gene(ID):
KAT6A(7994)
Sequence:
MVKLANPLYTEWILEAIKKVKKQKQRPSEERICNAVSSSHGLDRKTVLEQLELSVKDGTILKVSNKGLNSYKDPDNPGRIALPKPRNHGKLDNKQNVDWNKLIKRAVEGLAESGGSTLKSIERFLKGQKDVSALFGGSAASGFHQQLRLAIKRAIGHGRLLKDGPLYRLNTKATNVDGKESCESLSCLPPVSLLPHEKDKPVAEPIPICSFCLGTKEQNREKKPEELISCADCGNSGHPSCLKFSPELTVRVKALRWQCIECKTCSSCRDQGKNADNMLFCDSCDRGFHMECCDPPLTRMPKGMWICQICRPRKKGRKLLQKKAAQIKRRYTNPIGRPKNRLKKQNTVSKGPFSKVRTGPGRGRKRKITLSSQSASSSSEEGYLERIDGLDFCRDSNVSLKFNKKTKGLIDGLTKFFTPSPDGRKARGEVVDYSEQYRIRKRGNRKSSTSDWPTDNQDGWDGKQENEERLFGSQEIMTEKDMELFRDIQEQALQKVGVTGPPDPQVRCPSVIEFGKYEIHTWYSSPYPQEYSRLPKLYLCEFCLKYMKSRTILQQHMKKCGWFHPPANEIYRKNNISVFEVDGNVSTIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVLTQNDVKGCHLVGYFSKEKHCQQKYNVSCIMILPQYQRKGYGRFLIDFSYLLSKREGQAGSPEKPLSDLGRLSYMAYWKSVILECLYHQNDKQISIKKLSKLTGICPQDITSTLHHLRMLDFRSDQFVIIRREKLIQDHMAKLQLNLRPVDVDPECLRWTPVIVSNSVVSEEEEEEAEEGENEEPQCQERELEISVGKSVSHENKEQDSYSVESEKKPEVMAPVSSTRLSKQVLPHDSLPANSQPSRRGRWGRKNRKTQERFGDKDSKLLLEETSSAPQEQYGECGEKSEATQEQYTESEEQLVASEEQPSQDGKPDLPKRRLSEGVEPWRGQLKKSPEALKCRLTEGSERLPRRYSEGDRAVLRGFSESSEEEEEPESPRSSSPPILTKPTLKRKKPFLHRRRRVRKRKHHNSSVVTETISETTEVLDEPFEDSDSERPMPRLEPTFEIDEEEEEEDENELFPREYFRRLSSQDVLRCQSSSKRKSKDEEEDEESDDADDTPILKPVSLLRKRDVKNSPLEPDTSTPLKKKKGWPKGKSRKPIHWKKRPGRKPGFKLSREIMPVSTQACVIEPIVSIPKAGRKPKIQESEETVEPKEDMPLPEERKEEEEMQAEAEEAEEGEEEDAASSEVPAASPADSSNSPETETKEPEVEEEEEKPRVSEEQRQSEEEQQELEEPEPEEEEDAAAETAQNDDHDADDEDDGHLESTKKKELEEQPTREDVKEEPGVQESFLDANMQKSREKIKDKEETELDSEEEQPSHDTSVVSEQMAGSEDDHEEDSHTKEELIELKEEEEIPHSELDLETVQAVQSLTQEESSEHEGAYQDCEETLAACQTLQSYTQADEDPQMSMVEDCHASEHNSPISSVQSHPSQSVRSVSSPNVPALESGYTQISPEQGSLSAPSMQNMETSPMMDVPSVSDHSQQVVDSGFSDLGSIESTTENYENPSSYDSTMGGSICGNSSSQSSCSYGGLSSSSSLTQSSCVVTQQMASMGSSCSMMQQSSVQPAANCSIKSPQSCVVERPPSNQQQQPPPPPPQQPQPPPPQPQPAPQPPPPQQQPQQQPQPQPQQPPPPPPPQQQPPLSQCSMNNSFTPAPMIMEIPESGSTGNISIYERIPGDFGAGSYSQPSATFSLAKLQQLTNTIMDPHAMPYSHSPAVTSYATSVSLSNTGLAQLAPSHPLAGTPQAQATMTPPPNLASTTMNLTSPLLQCNMSATNIGIPHTQRLQGQMPVKGHISIRSKSAPLPSAAAHQQQLYGRSPSAVAMQAGPRALAVQRGMNMGVNLMPTPAYNVNSMNMNTLNAMNSYRMTQPMMNSSYHSNPAYMNQTAQYPMQMQMGMMGSQAYTQQPMQPNPHGNMMYTGPSHHSYMNAAGVPKQSLNGPYMRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
90
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92794 As Substrate

Site PTM Type Enzyme
S28 Phosphorylation
S116 Phosphorylation
T117 Phosphorylation
S132 Phosphorylation
S138 Phosphorylation
S141 Phosphorylation
S245 Phosphorylation
T332 Phosphorylation
T347 Phosphorylation
K350 Acetylation
K355 Acetylation
T369 Phosphorylation P31749 (AKT1)
S379 Phosphorylation
Y383 Phosphorylation
K415 Acetylation
K415 Ubiquitination
T418 Phosphorylation
S420 Phosphorylation
Y433 Phosphorylation
Y437 Phosphorylation
S447 Phosphorylation
S448 Phosphorylation
T449 Phosphorylation
S450 Phosphorylation
K463 Ubiquitination
S473 Phosphorylation
K480 Ubiquitination
K495 Ubiquitination
K604 Acetylation
S678 Phosphorylation
S787 Phosphorylation
S812 Phosphorylation
K815 Acetylation
S826 Phosphorylation
S828 Phosphorylation
S842 Phosphorylation
C902 S-Nitrosylation
T909 Phosphorylation
S941 Phosphorylation
S954 Phosphorylation
S974 Phosphorylation
S996 Phosphorylation
S999 Phosphorylation
S1001 Phosphorylation
K1007 Acetylation
K1014 Acetylation
T1042 Phosphorylation
S1052 Phosphorylation
S1054 Phosphorylation
S1089 Phosphorylation
S1090 Phosphorylation
S1104 Phosphorylation
S1113 Phosphorylation
T1119 Phosphorylation
S1126 Phosphorylation
S1136 Phosphorylation
T1142 Phosphorylation
S1143 Phosphorylation
T1144 Phosphorylation
K1147 Methylation
K1148 Methylation
K1174 Methylation
K1330 Methylation
Y1732 Phosphorylation
S1861 Phosphorylation
R1877 Methylation
R1889 Methylation
R2003 Methylation
R2004 Methylation

Research Backgrounds

Function:

Histone acetyltransferase that acetylates lysine residues in histone H3 and histone H4 (in vitro). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. May act as a transcriptional coactivator for RUNX1 and RUNX2. Acetylates p53/TP53 at 'Lys-120' and 'Lys-382' and controls its transcriptional activity via association with PML.

PTMs:

Autoacetylation at Lys-604 is required for proper function (By similarity). Autoacetylated.

Phosphorylation at Thr-369 by PKB/AKT1 inhibits its interaction with PML and negatively regulates its acetylation activity towards p53/TP53.

Subcellular Location:

Nucleus. Nucleus>Nucleolus. Nucleus>Nucleoplasm. Nucleus>PML body.
Note: Recruited into PML body after DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MOZ/MORF complex composed at least of ING5, KAT6A, KAT6B, MEAF6 and one of BRPF1, BRD1/BRPF2 and BRPF3. Interacts with RUNX1; phosphorylation of RUNX1 enhances the interaction. Interacts with RUNX2. Interacts with p53/TP53. Interacts with PML (isoform PML-4) and this interaction positively regulates its acetylation activity towards p53/TP53.

Family&Domains:

The N-terminus is involved in transcriptional activation while the C-terminus is involved in transcriptional repression.

Belongs to the MYST (SAS/MOZ) family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

References

1). The histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA binding winged helix domain. Nucleic Acids Research, 2023 (PubMed: 36537216) [IF=14.9]
2). Characterization of histone acetyltransferase KAT6A and transcriptional repressor SAMD1 – two proteins possessing a novel class of unmethylated DNA-binding winged helix domains. , 2023

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