Product: MYL6B Antibody
Catalog: DF9022
Description: Rabbit polyclonal antibody to MYL6B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Dog
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P14649
RRID: AB_2842218

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Dog(88%)
Clonality:
Polyclonal
Specificity:
MYL6B Antibody detects endogenous levels of total MYL6B.
RRID:
AB_2842218
Cite Format: Affinity Biosciences Cat# DF9022, RRID:AB_2842218.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MLC1sa; MYL 6B; Myl6b; MYL6B_HUMAN; Myosin alkali light chain 1 slow a; Myosin light chain 1 slow a; Myosin light chain 1 slow twitch muscle A isoform; Myosin light chain 1 slow-twitch muscle A isoform; Myosin light chain 6B alkali smooth muscle and non muscle; Myosin light chain 6B; Myosin light polypeptide 6B alkali smooth muscle and non muscle; Myosin light polypeptide 6B; Smooth muscle and non muscle myosin alkali light chain 6B; Smooth muscle and nonmuscle myosin light chain alkali 6B;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MPPKKDVPVKKPAGPSISKPAAKPAAAGAPPAKTKAEPAVPQAPQKTQEPPVDLSKVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTEEEVETVLAGHEDSNGCINYEAFLKHILSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
88
Pig
75
Horse
75
Rabbit
63
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14649 As Substrate

Site PTM Type Enzyme
S20 Phosphorylation
R78 Methylation
Y86 Phosphorylation
S87 Phosphorylation
T101 Phosphorylation
K107 Acetylation
K107 Ubiquitination
S114 Phosphorylation
Y146 Phosphorylation

Research Backgrounds

Function:

Regulatory light chain of myosin. Does not bind calcium.

Subunit Structure:

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.