MAGE1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of MAGE1 using K562 whole cell lysates.

DF9019 at 1/100 staining Mouse spleen tissue by IHC-P.

Product:	MAGE1 Antibody
Catalog:	DF9019
Description:	Rabbit polyclonal antibody to MAGE1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	103 kDa; 103kD(Calculated).
Uniprot:	Q9HCI5
RRID:	AB_2842215

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MAGE1 Antibody detects endogenous levels of total MAGE1.

RRID:

AB_2842215
Cite Format: Affinity Biosciences Cat# DF9019, RRID:AB_2842215.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI847422; Alpha-dystrobrevin-associated MAGE Protein; DAMAGE; HCA1; Hepatocellular carcinoma-associated protein 1; MAGE-E1 antigen; MAGE1_HUMAN; MAGEE1; Melanoma-associated antigen E1; mMage-e1; RGD1560259; RP23-82A12.1; RP4-596C3.1;

Immunogens

Immunogen:

Uniprot:

Q9HCI5(MAGE1_HUMAN) >>Visit HPA database.

Gene(ID):

MAGEE1(57692)

Sequence:

Q9HCI5 MAGE1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLVSQNSRRRRRRVAKATAHNSSWGEMQAPNAPGLPADVPGSDVPQGPSDSQILQGLCASEGPSTSVLPTSAEGPSTFVPPTISEASSASGQPTISEGPGTSVLPTPSEGLSTSGPPTISKGLCTSVTLAASEGRNTSRPPTSSEEPSTSVPPTASEVPSTSLPPTPGEGTSTSVPPTAYEGPSTSVVPTPDEGPSTSVLPTPGEGPGTSVPLAATEGLSTSVQATPDEGPSTSVPPTATEGLSTPVPPTRDEGPSTSVPATPGEGPSTSVLPAASDGQSISLVPTRGKGSSTSVPPTATEGLSTSVQPTAGEGSSTSVPPTPGGGLSTSVPPTATEELSTSVPPTPGEGPSTSVLPIPGEGLSTSVPPTASDGSDTSVPPTPGEGASTLVQPTAPDGPGSSVLPNPGEGPSTLFSSSASVDRNPSKCSLVLPSPRVTKASVDSDSEGPKGAEGPIEFEVLRDCESPNSISIMGLNTSRVAITLKPQDPMEQNVAELLQFLLVKDQSKYPIRESEMREYIVKEYRNQFPEILRRAAAHLECIFRFELRELDPEAHTYILLNKLGPVPFEGLEESPNGPKMGLLMMILGQIFLNGNQAKEAEIWEMLWRMGVQRERRLSIFGNPKRLLSVEFVWQRYLDYRPVTDCKPVEYEFFWGPRSHLETTKMKILKFMAKIYNKDPMDWPEKYNEALEEDAARAFAEGWQALPHFRRPFFEEAAAEVPSPDSEVSSYSSKYAPHSWPESRLESKARKLVQLFLLMDSTKLPIPKKGILYYIGRECSKVFPDLLNRAARTLNHVYGTELVVLDPRNHSYTLYNRREMEETEEIVDSPNRPGNNFLMQVLSFIFIMGNHARESAVWAFLRGLGVQAGRKHVITCRYLSQRYIDSLRVPDSDPVQYEFVWGPRARLETSKMKALRYVARIHRKEPQDWPQQYREAMEDEANRADVGHRQIFVHNFR

PTMs - Q9HCI5 As Substrate

Q9HCI5

Site	PTM Type	Source
S8	Phosphorylation	Uniprot
S430	Phosphorylation	Uniprot
S442	Phosphorylation	Uniprot
S445	Phosphorylation	Uniprot
S467	Phosphorylation	Uniprot
Y520	Phosphorylation	Uniprot
K523	Ubiquitination	Uniprot
Y525	Phosphorylation	Uniprot
K734	Ubiquitination	Uniprot
Y878	Phosphorylation	Uniprot
Y883	Phosphorylation	Uniprot
K911	Acetylation	Uniprot

Research Backgrounds

Q9HCI5_MAGE1_HUMAN

Function:

May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Subcellular Location:

Cytoplasm>Perinuclear region. Nucleus. Cell membrane.
Note: In the skeletal muscle, found at the postsynaptic membrane and is associated with a subset of myonuclei. May reside within nuclei and/or in perinuclear compartments. In peripheral nerves, colocalizes with DTNA in the Schwann cell membrane (By similarity).

Subunit Structure:

Interacts with DTNA (By similarity). Interacts with TRIM28.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MAGE1 Antibody - #DF9019