Cytokeratin 6 Antibody - #DF9011

(3)  
Product: Cytokeratin 6 Antibody
Catalog: DF9011
Description: Rabbit polyclonal antibody to Cytokeratin 6
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 60 kDa; 60kD(Calculated).
Uniprot: P02538 | P04259 | P48668
RRID: AB_2842207

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Cytokeratin 6 Antibody detects endogenous levels of total Cytokeratin 6.
RRID:
AB_2842207
Cite Format: Affinity Biosciences Cat# DF9011, RRID:AB_2842207.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 4; CK 5; CK 6A; CK 6B; CK 6C; CK 6D; CK 6E; CK4; CK5; CK6A; CK6B; CK6C; CK6D; CYK4; Cytokeratin 4; Cytokeratin 5; Cytokeratin 6A; Cytokeratin 6B; Cytokeratin 6C; Cytokeratin 6D; Cytokeratin 6E; DDD; K4; K5; K6A; K6a keratin; K6B; K6C; K6D; Keratin 5; Keratin 6A; Keratin 6B; Keratin 6C; Keratin 6D; Keratin 6E; Keratin K6h; Keratin, epidermal type II, K6A; Keratin, epidermal type II, K6C; Keratin, epidermal, type II, K6B; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6B; Keratin, type II cytoskeletal 6D; KRT5A; KRT6C; KRT6D; KRT6E; CK-6B; CK6B; Cytokeratin-6B; K2C6B_HUMAN; K6b; Keratin; Keratin epidermal type II K6B; Keratin like 1 (a type II keratin sequence); Keratin type II cytoskeletal 6B; Keratin-6B; KRT6B; KRTL1; PC2; PC4; type II cytoskeletal 6B; Type-II keratin Kb10; CK-6C; CK-6E; Cytokeratin-6C; Cytokeratin-6E; K2C6C_HUMAN; K6C; K6E; keratin 6C; Keratin; Keratin K6h; Keratin type II cytoskeletal 6C; Keratin-6C; KRT6C; KRT6E; type II cytoskeletal 6C; Type-II keratin Kb12;

Immunogens

Immunogen:
Uniprot:

P02538(K2C6A_HUMAN) >>Visit HPA database.

P04259(K2C6B_HUMAN) >>Visit HPA database.

P48668(K2C6C_HUMAN) >>Visit HPA database.

Gene(ID):
KRT6A(3853) | KRT6B(3854) | KRT6C(286887)
Expression:
P02538 K2C6A_HUMAN:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

P04259 K2C6B_HUMAN:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

P48668 K2C6C_HUMAN:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Sequence:
MASTSTTIRSHSSSRRGFSANSARLPGVSRSGFSSVSVSRSRGSGGLGGACGGAGFGSRSLYGLGGSKRISIGGGSCAISGGYGSRAGGSYGFGGAGSGFGFGGGAGIGFGLGGGAGLAGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPTIQRVRAEEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRGMQDLVEDFKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQVTAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSRKSYKH

MASTSTTIRSHSSSRRGFSANSARLPGVSRSGFSSISVSRSRGSGGLGGACGGAGFGSRSLYGLGGSKRISIGGGSCAISGGYGSRAGGSYGFGGAGSGFGFGGGAGIGFGLGGGAGLAGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPAIQRVRAEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDNIVGERGRLDSELRNMQDLVEDLKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQITAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRATGGGLSSVGGGSSTIKYTTTSSSSRKSYKH

MASTSTTIRSHSSSRRGFSANSARLPGVSRSGFSSISVSRSRGSGGLGGACGGAGFGSRSLYGLGGSKRISIGGGSCAISGGYGSRAGGSYGFGGAGSGFGFGGGAGIGFGLGGGAGLAGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPAIQRVRAEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDLKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQVTAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCASLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNVSVVQSTISSGYGGASGVGSGLGLGGGSSYSYGSGLGIGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSRKSYKH

PTMs - P02538/P04259/P48668 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S5 Phosphorylation
S10 Phosphorylation
S19 Phosphorylation
S22 Phosphorylation
S31 Phosphorylation
S34 Phosphorylation
S37 Phosphorylation
S41 Phosphorylation
R42 Methylation
S44 Phosphorylation
S58 Phosphorylation
S60 Phosphorylation
Y62 Phosphorylation
S67 Phosphorylation
S71 Phosphorylation
S76 Phosphorylation
S80 Phosphorylation
Y83 Phosphorylation
S85 Phosphorylation
K168 Ubiquitination
T169 Phosphorylation
K173 Acetylation
K173 Methylation
K173 Sumoylation
K173 Ubiquitination
S176 Phosphorylation
K180 Acetylation
K180 Ubiquitination
K194 Methylation
Y217 Phosphorylation
S237 Phosphorylation
K252 Methylation
K252 Ubiquitination
Y253 Phosphorylation
K259 Methylation
K259 Ubiquitination
Y278 Phosphorylation
T290 Phosphorylation
Y302 Phosphorylation
S332 Phosphorylation
Y341 Phosphorylation
S348 Phosphorylation
Y356 Phosphorylation
T358 Phosphorylation
Y360 Phosphorylation
T366 Phosphorylation
S393 Phosphorylation
S404 Phosphorylation
K426 Acetylation
Y448 Phosphorylation
R475 Methylation
S540 Phosphorylation
S541 Phosphorylation
S546 Phosphorylation
K550 Methylation
Y551 Phosphorylation
T552 Phosphorylation
S557 Phosphorylation
S558 Phosphorylation
S561 Phosphorylation
Site PTM Type Enzyme
S3 Phosphorylation
S5 Phosphorylation
S10 Phosphorylation
S19 Phosphorylation
S22 Phosphorylation
S31 Phosphorylation
S34 Phosphorylation
S37 Phosphorylation
S41 Phosphorylation
R42 Methylation
S44 Phosphorylation
S58 Phosphorylation
S60 Phosphorylation
Y62 Phosphorylation
S67 Phosphorylation
S71 Phosphorylation
S76 Phosphorylation
S80 Phosphorylation
Y83 Phosphorylation
S85 Phosphorylation
K168 Ubiquitination
T169 Phosphorylation
K173 Acetylation
K173 Methylation
K173 Sumoylation
K173 Ubiquitination
S176 Phosphorylation
K180 Acetylation
K180 Ubiquitination
K194 Methylation
Y217 Phosphorylation
S237 Phosphorylation
K252 Methylation
K252 Ubiquitination
Y253 Phosphorylation
K259 Methylation
K259 Ubiquitination
Y278 Phosphorylation
T290 Phosphorylation
Y302 Phosphorylation
S332 Phosphorylation
Y341 Phosphorylation
S348 Phosphorylation
Y356 Phosphorylation
T358 Phosphorylation
Y360 Phosphorylation
T366 Phosphorylation
S393 Phosphorylation
K426 Acetylation
Y448 Phosphorylation
R475 Methylation
S540 Phosphorylation
S541 Phosphorylation
S546 Phosphorylation
T548 Phosphorylation
Y551 Phosphorylation
S557 Phosphorylation
S558 Phosphorylation
S561 Phosphorylation
Site PTM Type Enzyme
S3 Phosphorylation
S5 Phosphorylation
S10 Phosphorylation
S19 Phosphorylation
S22 Phosphorylation
S31 Phosphorylation
S34 Phosphorylation
S37 Phosphorylation
S41 Phosphorylation
R42 Methylation
S44 Phosphorylation
S58 Phosphorylation
S60 Phosphorylation
Y62 Phosphorylation
S67 Phosphorylation
S71 Phosphorylation
S76 Phosphorylation
S80 Phosphorylation
Y83 Phosphorylation
S85 Phosphorylation
K168 Ubiquitination
T169 Phosphorylation
K173 Acetylation
K173 Methylation
K173 Sumoylation
K173 Ubiquitination
S176 Phosphorylation
K180 Acetylation
K180 Ubiquitination
K194 Ubiquitination
K204 Ubiquitination
Y217 Phosphorylation
S237 Phosphorylation
K252 Methylation
K252 Ubiquitination
Y253 Phosphorylation
K259 Methylation
K259 Ubiquitination
Y278 Phosphorylation
T290 Phosphorylation
Y302 Phosphorylation
S332 Phosphorylation
Y341 Phosphorylation
S348 Phosphorylation
Y356 Phosphorylation
T358 Phosphorylation
Y360 Phosphorylation
T366 Phosphorylation
S393 Phosphorylation
K426 Acetylation
Y448 Phosphorylation
R475 Methylation
S540 Phosphorylation
S541 Phosphorylation
S546 Phosphorylation
K550 Methylation
Y551 Phosphorylation
T552 Phosphorylation
S557 Phosphorylation
S558 Phosphorylation
S561 Phosphorylation

Research Backgrounds

Function:

Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

Tissue Specificity:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Subunit Structure:

Heterodimer of a type I and a type II keratin. KRT6 isomers associate with KRT16 and/or KRT17 (By similarity). Interacts with TCHP.

Family&Domains:

Belongs to the intermediate filament family.

Tissue Specificity:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Subunit Structure:

Heterodimer of a type I and a type II keratin. KRT6 isomers associate with KRT16 and/or KRT17.

Family&Domains:

Belongs to the intermediate filament family.

Tissue Specificity:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Subunit Structure:

Heterodimer of a type I and a type II keratin. KRT6 isomers associate with KRT16 and/or KRT17.

Family&Domains:

Belongs to the intermediate filament family.

Restrictive clause

 

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