K2C3 Antibody - #DF9010
(1)
| Product: | K2C3 Antibody |
| Catalog: | DF9010 |
| Description: | Rabbit polyclonal antibody to K2C3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Rabbit |
| Mol.Wt.: | 65 kDa; 64kD(Calculated). |
| Uniprot: | P12035 |
| RRID: | AB_2842206 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Rabbit(83%)
Clonality:
Polyclonal
Specificity:
K2C3 Antibody detects endogenous levels of total K2C3.
RRID:
AB_2842206
Cite Format: Affinity Biosciences Cat# DF9010, RRID:AB_2842206.
Cite Format: Affinity Biosciences Cat# DF9010, RRID:AB_2842206.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
65 kDa cytokeratin; CK-3; CK3; Cytokeratin-3; K2C3_HUMAN; K3; Keratin 3; Keratin; Keratin type II cytoskeletal 3; Keratin-3; KRT3; type II cytoskeletal 3; Type-II keratin Kb3;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- P12035 K2C3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRQASKTSGGGSQGFSGRSAVVSGSSRMSCVAHSGGAGGGAYGFRSGAGGFGSRSLYNLGGNKSISISVAAGGSRAGGFGGGRSSCAFAGGYGGGFGSGYGGGFGGGFGGGRGMGGGFGGAGGFGGAGGFGGAGGFGGPGGFGGSGGFGGPGSLGSPGGFGPGGFPGGIQEVTINQSLLQPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWNLLQQQGTSSISGTNNLEPLFENHINYLRSYLDNILGERGRLDSELKNMEDLVEDFKKKYEDEINKRTAAENEFVTLKKDVDSAYMNKVELQAKVDALIDEIDFLRTLYDAELSQMQSHISDTSVVLSMDNNRSLDLDSIIAEVRAQYEDIAQRSKAEAEALYQTKLGELQTTAGRHGDDLRNTKSEIIELNRMIQRLRAEIEGVKKQNANLQTAIAEAEQHGEMALKDANAKLQELQAALQQAKDDLARLLRDYQELMNVKLALDVEIATYRKLLEGEEYRMSGECPSAVSISVVSSSTTSASAGGYGGGYGGGMGGGLGGGFSAGGGSGSGFGRGGGGGIGGGFGGGSSGFSGGSGFGSISGARYGVSGGGFSSASNRGGSIKFSQSSQSSQRYSR
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
83
Horse
67
Sheep
67
Pig
0
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P12035 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| T204 | Phosphorylation | Uniprot | |
| K208 | Acetylation | Uniprot | |
| K208 | Methylation | Uniprot | |
| K208 | Sumoylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| K215 | Acetylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| Y393 | Phosphorylation | Uniprot | |
| T395 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| Y511 | Phosphorylation | Uniprot | |
| Y597 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S613 | Phosphorylation | Uniprot |
Research Backgrounds
Tissue Specificity:
Cornea specific.
Subunit Structure:
Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.
Family&Domains:
Belongs to the intermediate filament family.
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