Cytokeratin 2e Antibody - #DF9008

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Product: Cytokeratin 2e Antibody
Catalog: DF9008
Description: Rabbit polyclonal antibody to Cytokeratin 2e
Application: WB
Reactivity: Human
Prediction: Pig, Rabbit, Dog
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: P35908
RRID: AB_2842204

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(88%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 2e Antibody detects endogenous levels of total Cytokeratin 2e.
RRID:
AB_2842204
Cite Format: Affinity Biosciences Cat# DF9008, RRID:AB_2842204.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 2e; Cytokeratin-2e; Epithelial keratin-2e; K2e; Keratin type II cytoskeletal 2 epidermal; Keratin-2 epidermis; KRT2; KRT2A; KRT2E; Type-II keratin Kb2;

Immunogens

Immunogen:
Uniprot:

P35908(K22E_HUMAN) >>Visit HPA database.

Gene(ID):
KRT2(3849)
Expression:
P35908 K22E_HUMAN:

Expressed in the upper spinous and granular suprabasal layers of normal adult epidermal tissues from most body sites including thigh, breast nipple, foot sole, penile shaft and axilla. Not present in foreskin, squamous metaplasias and carcinomas. Expression in hypertrophic and keloid scars begins in the deepest suprabasal layer. Weakly expressed in normal gingiva and tongue, however expression is induced in benign keratoses of lingual mucosa and in mild-to-moderate oral dysplasia with orthokeratinization.

Sequence:
MSCQISCKSRGRGGGGGGFRGFSSGSAVVSGGSRRSTSSFSCLSRHGGGGGGFGGGGFGSRSLVGLGGTKSISISVAGGGGGFGAAGGFGGRGGGFGGGSSFGGGSGFSGGGFGGGGFGGGRFGGFGGPGGVGGLGGPGGFGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVKAQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKWELLQQMNVGTRPINLEPIFQGYIDSLKRYLDGLTAERTSQNSELNNMQDLVEDYKKKYEDEINKRTAAENDFVTLKKDVDNAYMIKVELQSKVDLLNQEIEFLKVLYDAEISQIHQSVTDTNVILSMDNSRNLDLDSIIAEVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELNRVIQRLQGEIAHVKKQCKNVQDAIADAEQRGEHALKDARNKLNDLEEALQQAKEDLARLLRDYQELMNVKLALDVEIATYRKLLEGEECRMSGDLSSNVTVSVTSSTISSNVASKAAFGGSGGRGSSSGGGYSSGSSSYGSGGRQSGSRGGSGGGGSISGGGYGSGGGSGGRYGSGGGSKGGSISGGGYGSGGGKHSSGGGSRGGSSSGGGYGSGGGGSSSVKGSSGEAFGSSVTFSFR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
88
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35908 As Substrate

Site PTM Type Enzyme
S62 Phosphorylation
S75 Phosphorylation
K183 Ubiquitination
T184 Phosphorylation
K188 Acetylation
K188 Methylation
K188 Sumoylation
K188 Ubiquitination
S191 Phosphorylation
K195 Acetylation
K195 Ubiquitination
Y264 Phosphorylation
K265 Acetylation
K267 Ubiquitination
S347 Phosphorylation
Y356 Phosphorylation
R490 Methylation
R524 Methylation
S526 Phosphorylation
S527 Phosphorylation
S528 Phosphorylation
S536 Phosphorylation
S546 Phosphorylation
S548 Phosphorylation
S557 Phosphorylation
Y563 Phosphorylation
Y573 Phosphorylation
S579 Phosphorylation
S597 Phosphorylation
S598 Phosphorylation
S602 Phosphorylation
S606 Phosphorylation
S607 Phosphorylation
S608 Phosphorylation
S614 Phosphorylation
S620 Phosphorylation
S621 Phosphorylation
T635 Phosphorylation
S637 Phosphorylation

Research Backgrounds

Function:

Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization. Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).

Tissue Specificity:

Expressed in the upper spinous and granular suprabasal layers of normal adult epidermal tissues from most body sites including thigh, breast nipple, foot sole, penile shaft and axilla. Not present in foreskin, squamous metaplasias and carcinomas. Expression in hypertrophic and keloid scars begins in the deepest suprabasal layer. Weakly expressed in normal gingiva and tongue, however expression is induced in benign keratoses of lingual mucosa and in mild-to-moderate oral dysplasia with orthokeratinization.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Associates with KRT10 (By similarity).

Family&Domains:

Belongs to the intermediate filament family.

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