Cytokeratin 9 Antibody - #DF9001

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Product: Cytokeratin 9 Antibody
Catalog: DF9001
Description: Rabbit polyclonal antibody to Cytokeratin 9
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 62 kDa; 62kD(Calculated).
Uniprot: P35527
RRID: AB_2842197

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(90%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 9 Antibody detects endogenous levels of total Cytokeratin 9.
RRID:
AB_2842197
Cite Format: Affinity Biosciences Cat# DF9001, RRID:AB_2842197.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 9; CK-9; Cytokeratin 9; Cytokeratin-9; Cytokeratin9; EPPK; K1C9_HUMAN; K9; Keratin 9; Keratin; Keratin type I cytoskeletal 9; Keratin-9; Keratin9; KRT 9; Krt9; Spermatid Perinuclear Ring Manchette Protein K9; type I cytoskeletal 9;

Immunogens

Immunogen:
Uniprot:

P35527(K1C9_HUMAN) >>Visit HPA database.

Gene(ID):
KRT9(3857)
Expression:
P35527 K1C9_HUMAN:

Expressed in the terminally differentiated epidermis of palms and soles.

Sequence:
MSCRQFSSSYLSRSGGGGGGGLGSGGSIRSSYSRFSSSGGGGGGGRFSSSSGYGGGSSRVCGRGGGGSFGYSYGGGSGGGFSASSLGGGFGGGSRGFGGASGGGYSSSGGFGGGFGGGSGGGFGGGYGSGFGGFGGFGGGAGGGDGGILTANEKSTMQELNSRLASYLDKVQALEEANNDLENKIQDWYDKKGPAAIQKNYSPYYNTIDDLKDQIVDLTVGNNKTLLDIDNTRMTLDDFRIKFEMEQNLRQGVDADINGLRQVLDNLTMEKSDLEMQYETLQEELMALKKNHKEEMSQLTGQNSGDVNVEINVAPGKDLTKTLNDMRQEYEQLIAKNRKDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLRHGVQELEIELQSQLSKKAALEKSLEDTKNRYCGQLQMIQEQISNLEAQITDVRQEIECQNQEYSLLLSIKMRLEKEIETYHNLLEGGQEDFESSGAGKIGLGGRGGSGGSYGRGSRGGSGGSYGGGGSGGGYGGGSGSRGGSGGSYGGGSGSGGGSGGGYGGGSGGGHSGGSGGGHSGGSGGNYGGGSGSGGGSGGGYGGGSGSRGGSGGSHGGGSGFGGESGGSYGGGEEASGSGGGYGGGSGKSSHS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Bovine
90
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35527 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S14 Phosphorylation
S24 Phosphorylation
S27 Phosphorylation
S30 Phosphorylation
S31 Phosphorylation
Y32 Phosphorylation
S36 Phosphorylation
S48 Phosphorylation
S49 Phosphorylation
S50 Phosphorylation
Y71 Phosphorylation
S166 Phosphorylation
K170 Ubiquitination
Y205 Phosphorylation
S297 Phosphorylation
S304 Phosphorylation
T320 Phosphorylation
S481 Phosphorylation
S484 Phosphorylation
Y485 Phosphorylation
S489 Phosphorylation
S493 Phosphorylation
S496 Phosphorylation
Y497 Phosphorylation
S502 Phosphorylation
S510 Phosphorylation
S512 Phosphorylation
S516 Phosphorylation
S519 Phosphorylation
Y520 Phosphorylation
S596 Phosphorylation
S607 Phosphorylation

Research Backgrounds

Function:

May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.

Tissue Specificity:

Expressed in the terminally differentiated epidermis of palms and soles.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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