ITGAD Antibody | Affinity Biosciences

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Western blot analysis ITGAD using COLO205 whole cell lysates.

Product:	ITGAD Antibody
Catalog:	DF8995
Description:	Rabbit polyclonal antibody to ITGAD
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	126 kDa; 127kD(Calculated).
Uniprot:	Q13349
RRID:	AB_2842191

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(82%), Horse(91%), Sheep(82%), Rabbit(91%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ITGAD Antibody detects endogenous levels of total ITGAD.

RRID:

AB_2842191
Cite Format: Affinity Biosciences Cat# DF8995, RRID:AB_2842191.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADB2; FLJ39841; integrin alpha D;

Immunogens

Immunogen:

Uniprot:

Q13349(ITAD_HUMAN) >>Visit HPA database.

Gene(ID):

ITGAD(3681)

Expression:

Q13349 ITAD_HUMAN:

Expressed moderately on myelomonocytic cell lines and subsets of peripheral blood leukocytes and strongly on tissue-specialized cells, including macrophages foam cells within atherosclerotic plaques, and on splenic red pulp macrophages.

Sequence:

Q13349 ITAD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTFGTVLLLSVLASYHGFNLDVEEPTIFQEDAGGFGQSVVQFGGSRLVVGAPLEVVAANQTGRLYDCAAATGMCQPIPLHIRPEAVNMSLGLTLAASTNGSRLLACGPTLHRVCGENSYSKGSCLLLGSRWEIIQTVPDATPECPHQEMDIVFLIDGSGSIDQNDFNQMKGFVQAVMGQFEGTDTLFALMQYSNLLKIHFTFTQFRTSPSQQSLVDPIVQLKGLTFTATGILTVVTQLFHHKNGARKSAKKILIVITDGQKYKDPLEYSDVIPQAEKAGIIRYAIGVGHAFQGPTARQELNTISSAPPQDHVFKVDNFAALGSIQKQLQEKIYAVEGTQSRASSSFQHEMSQEGFSTALTMDGLFLGAVGSFSWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQNLVLGAPRYQHTGKAVIFTQVSRQWRKKAEVTGTQIGSYFGASLCSVDVDSDGSTDLILIGAPHYYEQTRGGQVSVCPLPRGRVQWQCDAVLRGEQGHPWGRFGAALTVLGDVNEDKLIDVAIGAPGEQENRGAVYLFHGASESGISPSHSQRIASSQLSPRLQYFGQALSGGQDLTQDGLMDLAVGARGQVLLLRSLPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFEKNCGQDGLCEGDLGVTLSFSGLQTLTVGSSLELNVIVTVWNAGEDSYGTVVSLYYPAGLSHRRVSGAQKQPHQSALRLACETVPTEDEGLRSSRCSVNHPIFHEGSNGTFIVTFDVSYKATLGDRMLMRASASSENNKASSSKATFQLELPVKYAVYTMISRQEESTKYFNFATSDEKKMKEAEHRYRVNNLSQRDLAISINFWVPVLLNGVAVWDVVMEAPSQSLPCVSERKPPQHSDFLTQISRSPMLDCSIADCLQFRCDVPSFSVQEELDFTLKGNLSFGWVRETLQKKVLVVSVAEITFDTSVYSQLPGQEAFMRAQMEMVLEEDEVYNAIPIIMGSSVGALLLLALITATLYKLGFFKRHYKEMLEDKPEDTATFSGDDFSCVAPNVPLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Horse

Rabbit

Bovine

Sheep

Chicken

Pig

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13349 As Substrate

Q13349

Site	PTM Type	Source
Y119	Phosphorylation	Uniprot
S577	Phosphorylation	Uniprot
S614	Phosphorylation	Uniprot
S627	Phosphorylation	Uniprot
T886	Phosphorylation	Uniprot
S896	Phosphorylation	Uniprot
Y919	Phosphorylation	Uniprot
Y922	Phosphorylation	Uniprot
Y934	Phosphorylation	Uniprot

Research Backgrounds

Q13349_ITAD_HUMAN

Function:

Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. May play a role in the atherosclerotic process such as clearing lipoproteins from plaques and in phagocytosis of blood-borne pathogens, particulate matter, and senescent erythrocytes from the blood.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Alpha-D associates with beta-2.

Family&Domains:

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Belongs to the integrin alpha chain family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

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ITGAD Antibody - #DF8995