IL17F Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Hela cells, using IL17F Antibody.

Product:	IL17F Antibody
Catalog:	DF8981
Description:	Rabbit polyclonal antibody to IL17F
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Horse
Mol.Wt.:	18 kDa; 18kD(Calculated).
Uniprot:	Q96PD4
RRID:	AB_2842177

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(80%)

Clonality:

Polyclonal

Specificity:

IL17F Antibody detects endogenous levels of total IL17F.

RRID:

AB_2842177
Cite Format: Affinity Biosciences Cat# DF8981, RRID:AB_2842177.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CANDF6; Cytokine ML 1; Cytokine ML-1; IL 17F; IL 24; IL-17F; Il17f; IL17F_HUMAN; Interleukin 17F; Interleukin 24; Interleukin-17F; ML 1; ML1; Mutant IL 17F; OTTHUMP00000016602;

Immunogens

Immunogen:

Uniprot:

Q96PD4(IL17F_HUMAN) >>Visit HPA database.

Gene(ID):

IL17F(112744)

Expression:

Q96PD4 IL17F_HUMAN:

Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.

Sequence:

Q96PD4 IL17F_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTVKTLHGPAMVKYLLLSILGLAFLSEAAARKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96PD4 As Substrate

Q96PD4

Site	PTM Type	Enzyme	Source
N83	N-Glycosylation		Uniprot
S117	Phosphorylation		Uniprot

Research Backgrounds

Q96PD4_IL17F_HUMAN

Function:

Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover. Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis. Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation (By similarity).

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.

Subunit Structure:

Homodimer; disulfide-linked. Heterodimer with IL17A.

Family&Domains:

Belongs to the IL-17 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

IL17F Antibody - #DF8981