HSP74 Antibody - #DF8955

(2)  
Product: HSP74 Antibody
Catalog: DF8955
Description: Rabbit polyclonal antibody to HSP74
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 94kDa; 94kD(Calculated).
Uniprot: P34932
RRID: AB_2842151

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
HSP74 Antibody detects endogenous levels of total HSP74.
RRID:
AB_2842151
Cite Format: Affinity Biosciences Cat# DF8955, RRID:AB_2842151.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APG 2; Epididymis secretory sperm binding protein Li 5a; Heat shock 70 kDa protein 4; Heat shock 70 related protein APG 2; Heat shock 70-related protein APG-2; Heat shock protein, 110 kDa; HEL S 5a; HS24/P52; hsp70; Hsp70 RY; hsp70RY; HSP74_HUMAN; HSPA4; HSPH2; MGC131852; RY;

Immunogens

Immunogen:
Uniprot:

P34932(HSP74_HUMAN) >>Visit HPA database.

Gene(ID):
HSPA4(3308)
Sequence:
MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACISFGPKNRSIGAAAKSQVISNAKNTVQGFKRFHGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDCVVSVPCFYTDAERRSVMDATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQVSVCAFNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKIRALLRLSQECEKLKKLMSANASDLPLSIECFMNDVDVSGTMNRGKFLEMCNDLLARVEPPLRSVLEQTKLKKEDIYAVEIVGGATRIPAVKEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGSSDCEVFSKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIFSVSSASLVEVHKSEENEEPMETDQNAKEEEKMQVDQEEPHVEEQQQQTPAENKAESEEMETSQAGSKDKKMDQPPQAKKAKVKTSTVDLPIENQLLWQIDREMLNLYIENEGKMIMQDKLEKERNDAKNAVEEYVYEMRDKLSGEYEKFVSEDDRNSFTLKLEDTENWLYEDGEDQPKQVYVDKLAELKNLGQPIKIRFQESEERPKLFEELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCSPIISKPKPKVEPPKEEQKNAEQNGPVDGQGDNPGPQAAEQGTDTAVPSDSDKKLPEMDID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P34932 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
Y30 Phosphorylation
S31 Phosphorylation
R33 Methylation
C34 S-Nitrosylation
C38 S-Nitrosylation
K44 Ubiquitination
K53 Acetylation
K53 Ubiquitination
S54 Phosphorylation
K61 Ubiquitination
K68 Ubiquitination
S76 Phosphorylation
K84 Ubiquitination
S85 Phosphorylation
Y89 Phosphorylation
T96 Phosphorylation
T99 Phosphorylation
K102 Ubiquitination
T113 Phosphorylation
T114 Phosphorylation
T118 Phosphorylation
S123 Phosphorylation
K124 Ubiquitination
K126 Ubiquitination
K135 Ubiquitination
S155 Phosphorylation
Y184 Phosphorylation
K185 Ubiquitination
K194 Acetylation
K194 Ubiquitination
K221 Ubiquitination
T230 Phosphorylation
K235 Ubiquitination
C245 S-Nitrosylation
K250 Ubiquitination
R265 Methylation
K272 Acetylation
K272 Ubiquitination
S278 Phosphorylation
S287 Phosphorylation
K305 Ubiquitination
K329 Acetylation
K329 Ubiquitination
K332 Ubiquitination
Y336 Phosphorylation
K351 Ubiquitination
K356 Acetylation
K356 Ubiquitination
K360 Ubiquitination
S384 Phosphorylation
K388 Methylation
K388 Ubiquitination
S393 Phosphorylation
S408 Phosphorylation
S415 Phosphorylation
C417 S-Nitrosylation
K422 Acetylation
K422 Ubiquitination
S429 Phosphorylation
K430 Acetylation
K430 Ubiquitination
T433 Phosphorylation
Y435 Phosphorylation
K437 Ubiquitination
K466 Ubiquitination
S475 Phosphorylation
S476 Phosphorylation
K477 Ubiquitination
K517 Ubiquitination
T538 Phosphorylation
K543 Ubiquitination
S546 Phosphorylation
S552 Phosphorylation
S556 Phosphorylation
K557 Methylation
K557 Ubiquitination
K559 Methylation
K568 Ubiquitination
K573 Ubiquitination
T576 Phosphorylation
K603 Acetylation
K609 Ubiquitination
K618 Ubiquitination
Y624 Phosphorylation
Y626 Phosphorylation
K631 Acetylation
K631 Ubiquitination
S633 Phosphorylation
Y636 Phosphorylation
K638 Ubiquitination
S641 Phosphorylation
R645 Methylation
S647 Phosphorylation
Y660 Phosphorylation
K668 Ubiquitination
Y671 Phosphorylation
K674 Acetylation
K674 Ubiquitination
K679 Acetylation
K679 Ubiquitination
K686 Ubiquitination
S692 Phosphorylation
K697 Ubiquitination
K704 Ubiquitination
K711 Ubiquitination
K717 Ubiquitination
K719 Ubiquitination
Y723 Phosphorylation
K733 Ubiquitination
K736 Ubiquitination
K748 Ubiquitination
K754 Ubiquitination
S756 Phosphorylation
K773 Methylation
K773 Ubiquitination
S780 Phosphorylation
S784 Phosphorylation
K785 Ubiquitination
K789 Ubiquitination
K794 Ubiquitination
K798 Ubiquitination
S828 Phosphorylation
S830 Phosphorylation

Research Backgrounds

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with TJP1/ZO-1.

Family&Domains:

Belongs to the heat shock protein 70 family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.