GTSE1 Antibody - #DF8953
(1)
(5)
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
GTSE1 Antibody detects endogenous levels of total GTSE1.
RRID:
AB_2842149
Cite Format: Affinity Biosciences Cat# DF8953, RRID:AB_2842149.
Cite Format: Affinity Biosciences Cat# DF8953, RRID:AB_2842149.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
B99; B99 homolog; G 2 and S phase expressed 1; G two S phase expressed protein 1; GTSE 1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9NYZ3 GTSE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDPKKEDILLLADEKFDFDLSLSSSSANEDDEVFFGPFGHKERCIAASLELNNPVPEQPPLPTSESPFAWSPLAGEKFVEVYKEAHLLALHIESSSRNQAAQAAKPEDPRSQGVERFIQESKLKINLFEKEKEMKKSPTSLKRETYYLSDSPLLGPPVGEPRLLASSPALPSSGAQARLTRAPGPPHSAHALPRESCTAHAASQAATQRKPGTKLLLPRAASVRGRSIPGAAEKPKKEIPASPSRTKIPAEKESHRDVLPDKPAPGAVNVPAAGSHLGQGKRAIPVPNKLGLKKTLLKAPGSTSNLARKSSSGPVWSGASSACTSPAVGKAKSSEFASIPANSSRPLSNISKSGRMGPAMLRPALPAGPVGASSWQAKRVDVSELAAEQLTAPPSASPTQPQTPEGGGQWLNSSCAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSAWRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF
PTMs - Q9NYZ3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Sumoylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K123 | Sumoylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K143 | Sumoylation | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| R182 | Methylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K215 | Methylation | Uniprot | |
| S223 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S228 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K310 | Methylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K331 | Methylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S439 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S464 | Phosphorylation | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| T489 | Phosphorylation | Uniprot | |
| R492 | Methylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| T513 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| T529 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| T656 | Phosphorylation | Uniprot | |
| T675 | Phosphorylation | Uniprot | |
| T677 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression.
PTMs:
Phosphorylated in mitosis.
Subcellular Location:
Cytoplasm>Cytoskeleton.
Note: Associated with microtubules.
Research Fields
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
References
1). miR-181a-5p restrains non-small cell lung cancer cell invasion and migration by regulating the GTSE1/p53/NF-κB axis. CELLS TISSUES ORGANS, 2022
(PubMed: 35700709)
[IF=2.7]
Application: WB Species: Human Sample: NSCLC cells
Figure 1 miR-181a-5p was down-regulated and GTSE1 was up-regulated in NSCLC cells. A: expression of miR-181a5p detected by RT-qPCR; B: expression of GTSE1 detected by RT-qPCR; C: protein expression of GTSE1 detected by Western blot. Cell experiment was replicated 3 times. Data were described as mean ± standard deviation. Data comparisons between two groups were analyzed using the t test. The post hoc test was conducted using Tukey’s multiple comparisons test.
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