FGF21 Antibody - #DF8947

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Product: FGF21 Antibody
Catalog: DF8947
Description: Rabbit polyclonal antibody to FGF21
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 22 kDa, 16 kDa; 22kD(Calculated).
Uniprot: Q9NSA1
RRID: AB_2842143

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Rabbit(80%), Dog(92%)
Clonality:
Polyclonal
Specificity:
FGF21 Antibody detects endogenous levels of total FGF21.
RRID:
AB_2842143
Cite Format: Affinity Biosciences Cat# DF8947, RRID:AB_2842143.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FGF 21; FGF-21; Fgf21; FGF21_HUMAN; FGFL; Fibroblast growth factor 21; PRO10196; UNQ3115;

Immunogens

Immunogen:
Uniprot:

Q9NSA1(FGF21_HUMAN) >>Visit HPA database.

Gene(ID):
FGF21(26291)
Sequence:
MDSDETGFEHSGLWVSVLAGLLLGACQAHPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
92
Bovine
92
Dog
92
Rabbit
80
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via C-terminus) with KLB; this interaction is direct. Interacts with FGFR4.

Family&Domains:

Belongs to the heparin-binding growth factors family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness, 2022 [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 6. Effects ofL. plantarum Q16 on key proteins involved in hepatic energy metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.
2). Lycopene attenuates oxidative stress-induced hepatic dysfunction of insulin signal transduction: involvement of FGF21 and mitochondria. The Journal of Nutritional Biochemistry, 2022 (PubMed: 36057413) [IF=5.6]
3). Theabrownin ameliorates liver inflammation, oxidative stress, and fibrosis in MCD diet-fed C57BL/6J mice. Frontiers in Endocrinology, 2023 [IF=5.2]

Application: WB    Species: Mouse    Sample:

FIGURE 7 Theabrownin increases the expression of FGF21 and reduces the phosphorylation of MAPK p38 in MCD diet-fed mice. (A) IHC for FGF21. (B) FGF 21 protein levels from western blot. (C) Quantification of IHC positive area for FGF21. (D) Quantification of FGF 21 protein levels from western blot. (E) p38 protein level and p38 phosphorylation. (F) FGF21 mRNA level. (G) Quantification of p38 phosphorylation (normalized by total p38). AU, arbitrary units. n=5-11 mice per group. The data are mean ± s.e. (error bars). ***p < 0.001, MCS Ctrl vs. MCD Ctrl. †, p
4). Liraglutide regulates lipid metabolism via FGF21-LKB1-AMPK-ACC1 pathway in white adipose tissues and macrophage of type 2 diabetic mice. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 33640604) [IF=3.1]

Application: WB    Species: mice    Sample: White adipose tissue (WAT)

Fig. 2. Effects of LRG on adipocyte size and the protein expression in WAT of diabetic mice. (A) Adipocyte size of the mice; (B) Quantification of Fig. 2A; (C) Immunohistochemical assay of WAT; (D) Quantification of Fig. 2C; (E) Protein expression in WAT; (F) Quantification of Fig. 3E n ¼ 6 mice/group. #p < 0.05, ##p < 0.01,###p < 0.001 vs CON; *p < 0.05,**p < 0.01, ***p < 0.001 vs DM.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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