DNAJA1 Antibody - #DF8940

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Product: DNAJA1 Antibody
Catalog: DF8940
Description: Rabbit polyclonal antibody to DNAJA1
Application: WB IHC IF/ICC
Cited expt.: IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 44 kDa; 45kD(Calculated).
Uniprot: P31689
RRID: AB_2842136

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DNAJA1 Antibody detects endogenous levels of total DNAJA1.
RRID:
AB_2842136
Cite Format: Affinity Biosciences Cat# DF8940, RRID:AB_2842136.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DJ 2; DJ2; DjA1; DnaJ (Hsp40) homolog, subfamily A, member 1; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; DNAJ2; Dnaja1; DNJA1_HUMAN; hDJ 2; HDJ-2; HDJ2; Heat shock 40 kDa protein 4; heat shock protein DNAJ like 2; Heat shock protein J2; HSDJ; HSJ-2; HSJ2; HSPF4; Human DnaJ protein 2; NEDD7; Neural precursor cell expressed developmentally down regulated 7; OTTHUMP00000021193;

Immunogens

Immunogen:

A synthesized peptide derived from human DNAJA1, corresponding to a region within C-terminal amino acids.

Uniprot:

P31689(DNJA1_HUMAN) >>Visit HPA database.

Gene(ID):
DNAJA1(3301)
Expression:
P31689 DNJA1_HUMAN:

Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.

Sequence:
MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
92
Xenopus
75
Chicken
58
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.

Subcellular Location:

Membrane>Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm>Perinuclear region. Mitochondrion.
Note: Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

References

1). Construction and validation of a regulatory T cells-based classification of renal cell carcinoma: an integrated bioinformatic analysis and clinical cohort study. Cellular oncology (Dordrecht, Netherlands), 2024 (PubMed: 39714755) [IF=4.9]

Application: IHC    Species: human    Sample:

Fig. 5 IHC staining and scoring for classifying patients in Xinhua cohort based on RCC-TSC. A The flowchart of our study. Our clinical study was mainly composed of three analytic processes, including IHC staining for the tumor tissue of the 370 patients with kidney neoplasm, molecular diagnosis based on the RCC-TSC, and statistical validation. The inclusion and exclusion criteria were also shown in detail. Firstly, 370 patients with kidney neoplasm and received curative surgery from 2016–2018 were included. Secondly, due to the incompleteness of the IHC staining information (DNAJA1, DNAJB4, LAG3, Mki67), one patient was excluded. Thirdly, when performing Chi-square tests and Kaplan-Meier survival analysis, 71 (lacking OS censor and PFS censor) or 75 patients (lacking OS and PFS) were correspondingly excluded. Finally, when conducting the multivariate proportional hazards Cox regression analysis, 10 patients whose histopathological classifications were not KICH, KIRC, or KIRP were excluded. B The boxplots showed the relative expression in transcriptional level for the four markers we used for IHC staining and classification. MKi67 and LAG3, DNAJA1 and DNAJB3 respectively expressed highest in HIRC and LIRC. C The heatmap illustrated the information of the 370 patients in our retrospective cohort. D IHC slides demonstrated the differential staining results of DNAJA1, DNAJB4, LAG3, Mki67 in three RCC-TSC subtypes. Specifically, DNAJA1 and DNAJB4 both had relatively high, medium, and low staining score in LIRC, MIRC, and HIRC. While LAG3, Mki67 both had relatively high, medium, and low staining score in HIRC, MIRC, and LIRC. IHC, immunohistochemical; RCC-TSC, renal cell carcinoma-Treg states-related prognostic classification; OS, overall survival; PFS, progression-free survival; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LIRC, low immune infiltration RCC; MIRC, medium immune infiltration RCC; HIRC, high immune infiltration RCC

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