Product: ARRS Antibody
Catalog: DF8919
Description: Rabbit polyclonal antibody to ARRS
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 45 kDa; 45kD(Calculated).
Uniprot: P10523
RRID: AB_2842115

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(82%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
ARRS Antibody detects endogenous levels of total ARRS.
RRID:
AB_2842115
Cite Format: Affinity Biosciences Cat# DF8919, RRID:AB_2842115.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

48 kDa protein; Arrestin 1; Arrestin; ARRS_HUMAN; Retinal S antigen (48 KDa protein); Retinal S-antigen; Rod photoreceptor arrestin; RP47; S antigen; S antigen retina and pineal gland (arrestin); S arrestin; S-AG; S-arrestin; SAG;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P10523 ARRS_HUMAN:

Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level).

Sequence:
MAASGKTSKSEPNHVIFKKISRDKSVTIYLGNRDYIDHVSQVQPVDGVVLVDPDLVKGKKVYVTLTCAFRYGQEDIDVIGLTFRRDLYFSRVQVYPPVGAASTPTKLQESLLKKLGSNTYPFLLTFPDYLPCSVMLQPAPQDSGKSCGVDFEVKAFATDSTDAEEDKIPKKSSVRLLIRKVQHAPLEMGPQPRAEAAWQFFMSDKPLHLAVSLNKEIYFHGEPIPVTVTVTNNTEKTVKKIKAFVEQVANVVLYSSDYYVKPVAMEEAQEKVPPNSTLTKTLTLLPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDRTVLGILVSYQIKVKLTVSGFLGELTSSEVATEVPFRLMHPQPEDPAKESYQDANLVFEEFARHNLKDAGEAEEGKRDKNDVDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
91
Sheep
91
Rabbit
91
Pig
82
Dog
82
Xenopus
80
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10523 As Substrate

Site PTM Type Enzyme
K9 Acetylation
Y29 Phosphorylation
Y62 Phosphorylation
T64 Phosphorylation
T66 Phosphorylation
Y120 Phosphorylation
Y129 Phosphorylation
S143 Phosphorylation
Y254 Phosphorylation
Y258 Phosphorylation
S348 Phosphorylation
S349 Phosphorylation
T353 Phosphorylation

Research Backgrounds

Function:

Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (By similarity). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells.

Subcellular Location:

Cell projection>Cilium>Photoreceptor outer segment. Membrane>Peripheral membrane protein.
Note: Highly expressed in photoreceptor outer segments in light-exposed retina. Evenly distributed throughout rod photoreceptor cells in dark-adapted retina (By similarity). Predominantly dectected at the proximal region of photoreceptor outer segments, near disk membranes (PubMed:3720866).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level).

Subunit Structure:

Monomer. Homodimer. Homotetramer. Interacts with RHO (via the phosphorylated C-terminus).

Family&Domains:

The C-terminus interferes with binding to non-phosphorylated RHO. Interaction with phosphorylated RHO triggers displacement of the C-terminus and leads to a conformation change that mediates high-affinity RHO binding.

Belongs to the arrestin family.

Research Fields

· Organismal Systems > Sensory system > Phototransduction.

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