ARRS Antibody | Affinity Biosciences

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Western blot analysis of ARRS using HeLa whole cell lysates.

Product:	ARRS Antibody
Catalog:	DF8919
Description:	Rabbit polyclonal antibody to ARRS
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	45 kDa; 45kD(Calculated).
Uniprot:	P10523
RRID:	AB_2842115

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(82%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

ARRS Antibody detects endogenous levels of total ARRS.

RRID:

AB_2842115
Cite Format: Affinity Biosciences Cat# DF8919, RRID:AB_2842115.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

48 kDa protein; Arrestin 1; Arrestin; ARRS_HUMAN; Retinal S antigen (48 KDa protein); Retinal S-antigen; Rod photoreceptor arrestin; RP47; S antigen; S antigen retina and pineal gland (arrestin); S arrestin; S-AG; S-arrestin; SAG;

Immunogens

Immunogen:

Uniprot:

P10523(ARRS_HUMAN) >>Visit HPA database.

Gene(ID):

SAG(6295)

Expression:

P10523 ARRS_HUMAN:

Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level).

Sequence:

P10523 ARRS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAASGKTSKSEPNHVIFKKISRDKSVTIYLGNRDYIDHVSQVQPVDGVVLVDPDLVKGKKVYVTLTCAFRYGQEDIDVIGLTFRRDLYFSRVQVYPPVGAASTPTKLQESLLKKLGSNTYPFLLTFPDYLPCSVMLQPAPQDSGKSCGVDFEVKAFATDSTDAEEDKIPKKSSVRLLIRKVQHAPLEMGPQPRAEAAWQFFMSDKPLHLAVSLNKEIYFHGEPIPVTVTVTNNTEKTVKKIKAFVEQVANVVLYSSDYYVKPVAMEEAQEKVPPNSTLTKTLTLLPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDRTVLGILVSYQIKVKLTVSGFLGELTSSEVATEVPFRLMHPQPEDPAKESYQDANLVFEEFARHNLKDAGEAEEGKRDKNDVDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Rabbit

Pig

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10523 As Substrate

P10523

Site	PTM Type	Source
K9	Acetylation	Uniprot
Y29	Phosphorylation	Uniprot
Y62	Phosphorylation	Uniprot
T64	Phosphorylation	Uniprot
T66	Phosphorylation	Uniprot
Y120	Phosphorylation	Uniprot
Y129	Phosphorylation	Uniprot
S143	Phosphorylation	Uniprot
Y254	Phosphorylation	Uniprot
Y258	Phosphorylation	Uniprot
S348	Phosphorylation	Uniprot
S349	Phosphorylation	Uniprot
T353	Phosphorylation	Uniprot

Research Backgrounds

P10523_ARRS_HUMAN

Function:

Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (By similarity). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells.

Subcellular Location:

Cell projection>Cilium>Photoreceptor outer segment. Membrane>Peripheral membrane protein.
Note: Highly expressed in photoreceptor outer segments in light-exposed retina. Evenly distributed throughout rod photoreceptor cells in dark-adapted retina (By similarity). Predominantly dectected at the proximal region of photoreceptor outer segments, near disk membranes (PubMed:3720866).

Tissue Specificity:

Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level).

Subunit Structure:

Monomer. Homodimer. Homotetramer. Interacts with RHO (via the phosphorylated C-terminus).

Family&Domains:

The C-terminus interferes with binding to non-phosphorylated RHO. Interaction with phosphorylated RHO triggers displacement of the C-terminus and leads to a conformation change that mediates high-affinity RHO binding.

Belongs to the arrestin family.

Research Fields

· Organismal Systems > Sensory system > Phototransduction.

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ARRS Antibody - #DF8919