CDK3 Antibody - #DF8911

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Product: CDK3 Antibody
Catalog: DF8911
Description: Rabbit polyclonal antibody to CDK3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: Q00526
RRID: AB_2842107

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(83%), Bovine(82%), Sheep(82%), Rabbit(86%)
Clonality:
Polyclonal
Specificity:
CDK3 Antibody detects endogenous levels of total CDK3.
RRID:
AB_2842107
Cite Format: Affinity Biosciences Cat# DF8911, RRID:AB_2842107.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cdk 3; Cdk3; CDK3_HUMAN; CDKN3; Cell division kinase 3; Cell division protein kinase 3; Cyclin dependent kinase 3; Cyclin-dependent kinase 3; OTTHUMP00000206828; p36;

Immunogens

Immunogen:
Uniprot:

Q00526(CDK3_HUMAN) >>Visit HPA database.

Gene(ID):
CDK3(1018)
Expression:
Q00526 CDK3_HUMAN:

Expressed in cancer cell lines and glioblastoma tissue.

Sequence:
MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
86
Pig
83
Bovine
82
Sheep
82
Horse
75
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00526 As Substrate

Site PTM Type Enzyme
K9 Ubiquitination
T14 Phosphorylation Q00526 (CDK3)
Y15 Phosphorylation Q00526 (CDK3)
Y19 Phosphorylation Q00526 (CDK3)
K33 Ubiquitination
K59 Ubiquitination
T158 Phosphorylation
Y159 Phosphorylation
T160 Phosphorylation Q00526 (CDK3)
T198 Phosphorylation
S207 Phosphorylation Q00526 (CDK3)
Y236 Phosphorylation
S272 Phosphorylation

PTMs - Q00526 As Enzyme

Substrate Site Source
P03372 (ESR1) S104 Uniprot
P03372 (ESR1) S106 Uniprot
P03372 (ESR1) S118 Uniprot
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
P06400 (RB1) S807 Uniprot
P06400 (RB1) S811 Uniprot
P18846 (ATF1) S63 Uniprot
P43629-1 (KIR3DL1) S296 Uniprot
Q00526 (CDK3) T14 Uniprot
Q00526 (CDK3) Y15 Uniprot
Q00526 (CDK3) Y19 Uniprot
Q00526 (CDK3) T160 Uniprot
Q00526 (CDK3) S207 Uniprot
Q12800 (TFCP2) S309 Uniprot
Q5TKA1 (LIN9) T96 Uniprot
Q8TDN4-2 (CABLES1) S48 Uniprot
Q8TDN4 (CABLES1) S313 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.

Tissue Specificity:

Expressed in cancer cell lines and glioblastoma tissue.

Subunit Structure:

Interacts with CABLES1 and CABLES2 (By similarity). Interacts with ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

References

1). Overexpression of SMYD3 in Ovarian Cancer is Associated with Ovarian Cancer Proliferation and Apoptosis via Methylating H3K4 and H4K20. Journal of Cancer, 2019 (PubMed: 31417652) [IF=3.9]

Application: WB    Species: mouse    Sample: tumor

Figure 6.| Knockdown of SMYD3 inhibits ovarian cancer carcinogenesis in vivo.(F-G) The protein expression of SMYD3 and its downstream regulators CDKN3, CDKN2A, CDKN2B, CDC25A, BIRC3, CD40LG in the tumor tissues of vector control group and SMYD3 knockdown group.
2). CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2017 (PubMed: 28108217) [IF=3.6]

Application: WB    Species: human    Sample: H1299 cells

Figure 6. miR-150 suppresses CDK3 expression in lung cancer compared cells. SPC1and H1299 cells were transfected with miR-150 mimics and mimics NC for forty-eight hours, respectively. A. Western blot of CDK3 in SPC1and H1299 cells. B. qRT-PCR analysis of miR-150 in SPC1and H1299 cells. C. qRT-PCR analysis of CDK3 mRNA in SPC1and H1299 cells. Data are the means ± SEM of three independent experiments. *p<0.05, **p

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