Collagen IX alpha 2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from hela, using Collagen IX α2 Antibody.

DF8905 at 1/100 staining Mouse brain tissue by IHC-P.

Product:	Collagen IX alpha 2 Antibody
Catalog:	DF8905
Description:	Rabbit polyclonal antibody to Collagen IX alpha 2
Application:	WB IHC
Reactivity:	Human, Mouse, Monkey
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	65 kDa; 65kD(Calculated).
Uniprot:	Q14055
RRID:	AB_2842101

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Monkey

Prediction:

Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(90%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Collagen IX alpha 2 Antibody detects endogenous levels of total Collagen IX alpha 2.

RRID:

AB_2842101
Cite Format: Affinity Biosciences Cat# DF8905, RRID:AB_2842101.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q14055(CO9A2_HUMAN) >>Visit HPA database.

Gene(ID):

COL9A2(1298)

Sequence:

Q14055 CO9A2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAATASPRSLLVLLQVVVLALAQIRGPPGERGPPGPPGPPGVPGSDGIDGDNGPPGKAGPPGPKGEPGKAGPDGPDGKPGIDGLTGAKGEPGPMGIPGVKGQPGLPGPPGLPGPGFAGPPGPPGPVGLPGEIGIRGPKGDPGPDGPSGPPGPPGKPGRPGTIQGLEGSADFLCPTNCPPGMKGPPGLQGVKGHAGKRGILGDPGHQGKPGPKGDVGASGEQGIPGPPGPQGIRGYPGMAGPKGETGPHGYKGMVGAIGATGPPGEEGPRGPPGRAGEKGDEGSPGIRGPQGITGPKGATGPPGINGKDGTPGTPGMKGSAGQAGQPGSPGHQGLAGVPGQPGTKGGPGDQGEPGPQGLPGFSGPPGKEGEPGPRGEIGPQGIMGQKGDQGERGPVGQPGPQGRQGPKGEQGPPGIPGPQGLPGVKGDKGSPGKTGPRGKVGDPGVAGLPGEKGEKGESGEPGPKGQQGVRGEPGYPGPSGDAGAPGVQGYPGPPGPRGLAGNRGVPGQPGRQGVEGRDATDQHIVDVALKMLQEQLAEVAVSAKREALGAVGMMGPPGPPGPPGYPGKQGPHGHPGPRGVPGIVGAVGQIGNTGPKGKRGEKGDPGEVGRGHPGMPGPPGIPGLPGRPGQAINGKDGDRGSPGAPGEAGRPGLPGPVGLPGFCEPAACLGASAYASARLTEPGSIKGP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14055 As Substrate

Q14055

Site	PTM Type	Source
S7	Phosphorylation	Uniprot
S10	Phosphorylation	Uniprot
K252	Acetylation	Uniprot
K279	Acetylation	Uniprot
S431	Phosphorylation	Uniprot
S459	Phosphorylation	Uniprot

Research Backgrounds

Q14055_CO9A2_HUMAN

Function:

Structural component of hyaline cartilage and vitreous of the eye.

PTMs:

Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links.

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Subunit Structure:

Heterotrimer of an alpha 1(IX), an alpha 2(IX) and an alpha 3(IX) chain (By similarity). The chains are linked to each other by interchain disulfide bonds (By similarity). Trimers are also cross-linked via hydroxylysines (By similarity).

Family&Domains:

Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Digestive system > Protein digestion and absorption.

Restrictive clause

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For Research Use Only.
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Collagen IX alpha 2 Antibody - #DF8905