AK5 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Mouse brain, using AK5 Antibody.

Product:	AK5 Antibody
Catalog:	DF8898
Description:	Rabbit polyclonal antibody to AK5
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.:	22 kDa; 63kD(Calculated).
Uniprot:	Q9Y6K8
RRID:	AB_2842094

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

AK5 Antibody detects endogenous levels of total AK5.

RRID:

AB_2842094
Cite Format: Affinity Biosciences Cat# DF8898, RRID:AB_2842094.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Adenylate kinase 5; Adenylate kinase 6; Adenylate kinase isoenzyme 5; AK 5; AK5; AK6; ATP AMP transphosphorylase 5; ATP-AMP transphosphorylase 5; KAD5; KAD5_HUMAN; MGC33326;

Immunogens

Immunogen:

Uniprot:

Q9Y6K8(KAD5_HUMAN) >>Visit HPA database.

Gene(ID):

AK5(26289)

Expression:

Q9Y6K8 KAD5_HUMAN:

Brain specific.

Sequence:

Q9Y6K8 KAD5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNTNDAKEYLARREIPQLFESLLNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKWDTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGGPGSGKGTQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEIKQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKINAEGTPEDVFLQLCTAIDSIF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

Pig

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6K8 As Substrate

Q9Y6K8

Site	PTM Type	Source
Y37	Phosphorylation	Uniprot
Y92	Phosphorylation	Uniprot
S105	Phosphorylation	Uniprot
S108	Phosphorylation	Uniprot
S143	Phosphorylation	Uniprot
K179	Acetylation	Uniprot
S181	Phosphorylation	Uniprot
K185	Acetylation	Uniprot
Y521	Phosphorylation	Uniprot
Y522	Phosphorylation	Uniprot
Y532	Phosphorylation	Uniprot

Research Backgrounds

Q9Y6K8_KAD5_HUMAN

Function:

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Brain specific.

Subunit Structure:

Monomer. Interacts with YWHAZ.

Family&Domains:

Belongs to the adenylate kinase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Thiamine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AK5 Antibody - #DF8898