THRAP3 Antibody - #DF8893

(2)  
Product: THRAP3 Antibody
Catalog: DF8893
Description: Rabbit polyclonal antibody to THRAP3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 109kDa; 109kD(Calculated).
Uniprot: Q9Y2W1
RRID: AB_2842089

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
THRAP3 Antibody detects endogenous levels of total THRAP3.
RRID:
AB_2842089
Cite Format: Affinity Biosciences Cat# DF8893, RRID:AB_2842089.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ22082; Thrap3; Thyroid hormone receptor associated protein 3; Thyroid hormone receptor-associated protein 3; Thyroid hormone receptor-associated protein complex 150 kDa component; Thyroid hormone receptor-associated protein complex; TR150_HUMAN; Trap150;

Immunogens

Immunogen:
Uniprot:

Q9Y2W1(TR150_HUMAN) >>Visit HPA database.

Gene(ID):
THRAP3(9967)
Expression:
Q9Y2W1 TR150_HUMAN:

Ubiquitous.

Sequence:
MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSLSRSRKRRLSSRSRSRSYSPAHNRERNHPRVYQNRDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYSPRRGRSRSRSPKRRSPSPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPSQAAGDNQGDEAKEQTFSGGTSQDTKASESSKPWPDATYGTGSASRASAVSELSPRERSPALKSPLQSVVVRRRSPRPSPVPKPSPPLSSTSQMGSTLPSGAGYQSGTHQGQFDHGSGSLSPSKKSPVGKSPPSTGSTYGSSQKEESAASGGAAYTKRYLEEQKTENGKDKEQKQTNTDKEKIKEKGSFSDTGLGDGKMKSDSFAPKTDSEKPFRGSQSPKRYKLRDDFEKKMADFHKEEMDDQDKDKAKGRKESEFDDEPKFMSKVIGANKNQEEEKSGKWEGLVYAPPGKEKQRKTEELEEESFPERSKKEDRGKRSEGGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESRDKLGAKGDFPTGKSSFSITREAQVNVRMDSFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQHIVTIVHHVKEHHFGSSGMTLHERFTKYLKRGTEQEAAKNKKSPEIHRRIDISPSTFRKHGLAHDEMKSPREPGYKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSAEKTEKTHKGSKKQKKHRRARDRSRSSSSSSQSSHSYKAEEYTEETEEREESTTGFDKSRLGTKDFVGPSERGGGRARGTFQFRARGRGWGRGNYSGNNNNNSNNDFQKRNREEEWDPEYTPKSKKYYLHDDREGEGSDKWVSRGRGRGAFPRGRGRFMFRKSSTSPKWAHDKFSGEEGEIEDDESGTENREEKDNIQPTTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2W1 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S24 Phosphorylation
S26 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S38 Phosphorylation
S46 Phosphorylation
S47 Phosphorylation
S51 Phosphorylation
S53 Phosphorylation
Y54 Phosphorylation
S55 Phosphorylation
R62 Methylation
R66 Methylation
Y68 Phosphorylation P12931 (SRC)
R71 Methylation
R74 Methylation
R78 Methylation
R81 Methylation
R87 Methylation
R91 Methylation
R101 Methylation
Y107 Phosphorylation P12931 (SRC)
R108 Methylation
Y114 Phosphorylation
R115 Methylation
Y118 Phosphorylation P12931 (SRC)
S119 Phosphorylation
S134 Phosphorylation
S136 Phosphorylation
S139 Phosphorylation
S143 Phosphorylation
S149 Phosphorylation
S150 Phosphorylation
S151 Phosphorylation
S154 Phosphorylation
S158 Phosphorylation
S163 Phosphorylation
S164 Phosphorylation
K178 Acetylation
S182 Phosphorylation
S183 Phosphorylation
S184 Phosphorylation
S187 Phosphorylation
S190 Phosphorylation
K202 Methylation
K202 Sumoylation
K202 Ubiquitination
S207 Phosphorylation
T210 Phosphorylation
S211 Phosphorylation
T214 Phosphorylation
S217 Phosphorylation
S219 Phosphorylation
S220 Phosphorylation
K221 Acetylation
K221 Ubiquitination
T227 Phosphorylation
Y228 Phosphorylation
T230 Phosphorylation
S232 Phosphorylation
S234 Phosphorylation
S237 Phosphorylation
S240 Phosphorylation
S243 Phosphorylation
S248 Phosphorylation
K252 Methylation
S253 Phosphorylation
S257 Phosphorylation
S264 Phosphorylation
S268 Phosphorylation
S274 Phosphorylation
S295 Phosphorylation
T297 Phosphorylation
S310 Phosphorylation
S312 Phosphorylation
S315 Phosphorylation
S320 Phosphorylation
S323 Phosphorylation
T324 Phosphorylation
S326 Phosphorylation
T327 Phosphorylation
Y328 Phosphorylation
S330 Phosphorylation
S331 Phosphorylation
S336 Phosphorylation
S339 Phosphorylation
Y344 Phosphorylation P12931 (SRC)
T345 Phosphorylation
Y348 Phosphorylation
T365 Phosphorylation
T367 Phosphorylation
S377 Phosphorylation
S379 Phosphorylation
T381 Phosphorylation
K387 Acetylation
K387 Sumoylation
S390 Phosphorylation
S392 Phosphorylation
T397 Phosphorylation
S399 Phosphorylation
K401 Acetylation
S406 Phosphorylation
S408 Phosphorylation
Y412 Phosphorylation
K420 Acetylation
K427 Sumoylation
K442 Methylation
S444 Phosphorylation
K451 Sumoylation
K451 Ubiquitination
K455 Acetylation
K455 Ubiquitination
K461 Sumoylation
K467 Sumoylation
S468 Phosphorylation
K470 Acetylation
K470 Sumoylation
K470 Ubiquitination
Y476 Phosphorylation
K481 Acetylation
K483 Acetylation
K486 Sumoylation
T487 Phosphorylation
S494 Phosphorylation
S499 Phosphorylation
K500 Methylation
S508 Phosphorylation
R513 Methylation
K519 Acetylation
K519 Ubiquitination
Y526 Phosphorylation
K527 Acetylation
S534 Phosphorylation
S535 Phosphorylation
K551 Ubiquitination
K558 Acetylation
K558 Ubiquitination
S560 Phosphorylation
S562 Phosphorylation
S575 Phosphorylation
S584 Phosphorylation
S600 Phosphorylation
S619 Phosphorylation
S622 Phosphorylation
S624 Phosphorylation
T633 Phosphorylation
K639 Acetylation
Y657 Phosphorylation
T662 Phosphorylation
S672 Phosphorylation
S682 Phosphorylation
S684 Phosphorylation
T685 Phosphorylation
S698 Phosphorylation
Y704 Phosphorylation
K705 Sumoylation
K709 Acetylation
Y710 Phosphorylation
K711 Methylation
K711 Sumoylation
S737 Phosphorylation
S740 Phosphorylation
S743 Phosphorylation
S746 Phosphorylation
S747 Phosphorylation
S749 Phosphorylation
S753 Phosphorylation
K756 Sumoylation
K759 Sumoylation
S779 Phosphorylation
S784 Phosphorylation
Y795 Phosphorylation
T796 Phosphorylation
T799 Phosphorylation
R802 Methylation
S805 Phosphorylation
T806 Phosphorylation
T807 Phosphorylation
K811 Acetylation
K811 Ubiquitination
S823 Phosphorylation
R825 Methylation
R829 Methylation
R831 Methylation
T833 Phosphorylation
R837 Methylation
R839 Methylation
R841 Methylation
R845 Methylation
Y873 Phosphorylation
T874 Phosphorylation P24941 (CDK2)
K876 Ubiquitination
S877 Phosphorylation
Y880 Phosphorylation
Y881 Phosphorylation
R886 Methylation
S891 Phosphorylation
R897 Methylation
R899 Methylation
R906 Methylation
R908 Methylation
R910 Methylation
S916 Phosphorylation
S917 Phosphorylation
T918 Phosphorylation
S919 Phosphorylation
K926 Acetylation
S928 Phosphorylation
S939 Phosphorylation
T941 Phosphorylation
T953 Phosphorylation
T954 Phosphorylation

Research Backgrounds

Function:

Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-ARNTL/BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes.

PTMs:

ADP-ribosylation during genotoxic stress promotes accumulation in nuclear speckles.

Subcellular Location:

Nucleus. Nucleus>Nucleoplasm. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Associated with the large multiprotein complex TRAP (Mediator complex-like). Interacts with SFPQ; the interaction is dependent on SFPQ phosphorylation at 'Thr-687' and inhibits binding of SFPQ to an ESS1 exonic splicing silencer element-containing RNA. Interacts with NXF1. Component of the SNARP complex which consists at least of SNIP1, SNW1, THRAP3, BCLAF1 and PNN. Associated with spliced mRNP complexes. Interacts with HELZ2 and PPARG. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, KIAA1429, RBM15, BCLAF1 and THRAP3.

Family&Domains:

Belongs to the BCLAF1/THRAP3 family.

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