WBP7 Antibody - #DF8886

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Product: WBP7 Antibody
Catalog: DF8886
Description: Rabbit polyclonal antibody to WBP7
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 294kDa; 294kD(Calculated).
Uniprot: Q9UMN6
RRID: AB_2842082

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
WBP7 Antibody detects endogenous levels of total WBP7.
RRID:
AB_2842082
Cite Format: Affinity Biosciences Cat# DF8886, RRID:AB_2842082.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Histone-lysine N-methyltransferase 2B; HRX2; KIAA0304 gene product; KMT2B; KMT2B_HUMAN; Likely ortholog of mouse WW domain binding protein 7; Lysine N-methyltransferase 2B; mixed lineage leukemia 4; Mixed lineage leukemia gene homolog 2 protein; MLL2; myeloid lymphoid leukemia 4; Myeloid lymphoid or mixed lineage leukemia protein 4; Myeloid/lymphoid or mixed-lineage leukemia protein 4; Trithorax homolog 2; trithorax homologue 2; TRX2; WBP-7; WBP7; WW domain-binding protein 7;

Immunogens

Immunogen:
Uniprot:

Q9UMN6(KMT2B_HUMAN) >>Visit HPA database.

Gene(ID):
KMT2B(9757)
Expression:
Q9UMN6 KMT2B_HUMAN:

Widely expressed. Highest levels in testis. Also found in brain with higher expression in the cerebellum than in any other region, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.

Sequence:
MAAAAGGGSCPGPGSARGRFPGRPRGAGGGGGRGGRGNGAERVRVALRRGGGATGPGGAEPGEDTALLRLLGLRRGLRRLRRLWAGPRVQRGRGRGRGRGWGPSRGCVPEEESSDGESDEEEFQGFHSDEDVAPSSLRSALRSQRGRAPRGRGRKHKTTPLPPPRLADVAPTPPKTPARKRGEEGTERMVQALTELLRRAQAPQAPRSRACEPSTPRRSRGRPPGRPAGPCRRKQQAVVVAEAAVTIPKPEPPPPVVPVKHQTGSWKCKEGPGPGPGTPRRGGQSSRGGRGGRGRGRGGGLPFVIKFVSRAKKVKMGQLSLGLESGQGQGQHEESWQDVPQRRVGSGQGGSPCWKKQEQKLDDEEEEKKEEEEKDKEGEEKEERAVAEEMMPAAEKEEAKLPPPPLTPPAPSPPPPLPPPSTSPPPPLCPPPPPPVSPPPLPSPPPPPAQEEQEESPPPVVPATCSRKRGRPPLTPSQRAEREAARAGPEGTSPPTPTPSTATGGPPEDSPTVAPKSTTFLKNIRQFIMPVVSARSSRVIKTPRRFMDEDPPKPPKVEVSPVLRPPITTSPPVPQEPAPVPSPPRAPTPPSTPVPLPEKRRSILREPTFRWTSLTRELPPPPPAPPPPPAPSPPPAPATSSRRPLLLRAPQFTPSEAHLKIYESVLTPPPLGAPEAPEPEPPPADDSPAEPEPRAVGRTNHLSLPRFAPVVTTPVKAEVSPHGAPALSNGPQTQAQLLQPLQALQTQLLPQALPPPQPQLQPPPSPQQMPPLEKARIAGVGSLPLSGVEEKMFSLLKRAKVQLFKIDQQQQQKVAASMPLSPGGQMEEVAGAVKQISDRGPVRSEDESVEAKRERPSGPESPVQGPRIKHVCRHAAVALGQARAMVPEDVPRLSALPLRDRQDLATEDTSSASETESVPSRSRRGKVEAAGPGGESEPTGSGGTLAHTPRRSLPSHHGKKMRMARCGHCRGCLRVQDCGSCVNCLDKPKFGGPNTKKQCCVYRKCDKIEARKMERLAKKGRTIVKTLLPWDSDESPEASPGPPGPRRGAGAGGPREEVVAHPGPEEQDSLLQRKSARRCVKQRPSYDIFEDSDDSEPGGPPAPRRRTPRENELPLPEPEEQSRPRKPTLQPVLQLKARRRLDKDALAPGPFASFPNGWTGKQKSPDGVHRVRVDFKEDCDLENVWLMGGLSVLTSVPGGPPMVCLLCASKGLHELVFCQVCCDPFHPFCLEEAERPLPQHHDTWCCRRCKFCHVCGRKGRGSKHLLECERCRHAYHPACLGPSYPTRATRKRRHWICSACVRCKSCGATPGKNWDVEWSGDYSLCPRCTQLYEKGNYCPICTRCYEDNDYESKMMQCAQCDHWVHAKCEGLSDEDYEILSGLPDSVLYTCGPCAGAAQPRWREALSGALQGGLRQVLQGLLSSKVVGPLLLCTQCGPDGKQLHPGPCGLQAVSQRFEDGHYKSVHSFMEDMVGILMRHSEEGETPDRRAGGQMKGLLLKLLESAFGWFDAHDPKYWRRSTRLPNGVLPNAVLPPSLDHVYAQWRQQEPETPESGQPPGDPSAAFQGKDPAAFSHLEDPRQCALCLKYGDADSKEAGRLLYIGQNEWTHVNCAIWSAEVFEENDGSLKNVHAAVARGRQMRCELCLKPGATVGCCLSSCLSNFHFMCARASYCIFQDDKKVFCQKHTDLLDGKEIVNPDGFDVLRRVYVDFEGINFKRKFLTGLEPDAINVLIGSIRIDSLGTLSDLSDCEGRLFPIGYQCSRLYWSTVDARRRCWYRCRILEYRPWGPREEPAHLEAAEENQTIVHSPAPSSEPPGGEDPPLDTDVLVPGAPERHSPIQNLDPPLRPDSGSAPPPAPRSFSGARIKVPNYSPSRRPLGGVSFGPLPSPGSPSSLTHHIPTVGDPDFPAPPRRSRRPSPLAPRPPPSRWASPPLKTSPQLRVPPPTSVVTALTPTSGELAPPGPAPSPPPPEDLGPDFEDMEVVSGLSAADLDFAASLLGTEPFQEEIVAAGAMGSSHGGPGDSSEEESSPTSRYIHFPVTVVSAPGLAPSATPGAPRIEQLDGVDDGTDSEAEAVQQPRGQGTPPSGPGVVRAGVLGAAGDRARPPEDLPSEIVDFVLKNLGGPGDGGAGPREESLPPAPPLANGSQPSQGLTASPADPTRTFAWLPGAPGVRVLSLGPAPEPPKPATSKIILVNKLGQVFVKMAGEGEPVPPPVKQPPLPPTISPTAPTSWTLPPGPLLGVLPVVGVVRPAPPPPPPPLTLVLSSGPASPPRQAIRVKRVSTFSGRSPPAPPPYKAPRLDEDGEASEDTPQVPGLGSGGFSRVRMKTPTVRGVLDLDRPGEPAGEESPGPLQERSPLLPLPEDGPPQVPDGPPDLLLESQWHHYSGEASSSEEEPPSPDDKENQAPKRTGPHLRFEISSEDGFSVEAESLEGAWRTLIEKVQEARGHARLRHLSFSGMSGARLLGIHHDAVIFLAEQLPGAQRCQHYKFRYHQQGEGQEEPPLNPHGAARAEVYLRKCTFDMFNFLASQHRVLPEGATCDEEEDEVQLRSTRRATSLELPMAMRFRHLKKTSKEAVGVYRSAIHGRGLFCKRNIDAGEMVIEYSGIVIRSVLTDKREKFYDGKGIGCYMFRMDDFDVVDATMHGNAARFINHSCEPNCFSRVIHVEGQKHIVIFALRRILRGEELTYDYKFPIEDASNKLPCNCGAKRCRRFLN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UMN6 As Substrate

Site PTM Type Enzyme
A2 Acetylation
R17 Methylation
R49 Methylation
T159 Phosphorylation
T172 Phosphorylation
T176 Phosphorylation
K180 Methylation
R181 Methylation
R188 Methylation
S214 Phosphorylation
S219 Phosphorylation
R222 Methylation
T278 Phosphorylation
R280 Methylation
S286 Phosphorylation
S346 Phosphorylation
S351 Phosphorylation
T475 Phosphorylation
T496 Phosphorylation
S500 Phosphorylation
T501 Phosphorylation
S510 Phosphorylation
K541 Methylation
T542 Phosphorylation
S582 Phosphorylation
T588 Phosphorylation
S602 Phosphorylation
S632 Phosphorylation
T653 Phosphorylation
S703 Phosphorylation
T713 Phosphorylation
S786 Phosphorylation
K797 Methylation
K797 Ubiquitination
S821 Phosphorylation
S844 Phosphorylation
S857 Phosphorylation
S861 Phosphorylation
T906 Phosphorylation
S910 Phosphorylation
S913 Phosphorylation
K926 Acetylation
S936 Phosphorylation
S941 Phosphorylation
T948 Phosphorylation
S1032 Phosphorylation
S1035 Phosphorylation
S1039 Phosphorylation
S1069 Phosphorylation
S1085 Phosphorylation
S1092 Phosphorylation
S1095 Phosphorylation
S1122 Phosphorylation
T1128 Phosphorylation
K1161 Methylation
S1164 Phosphorylation
Y1275 Phosphorylation
Y1284 Phosphorylation
S1519 Phosphorylation
T1550 Phosphorylation
K1567 Acetylation
K1692 Ubiquitination
Y1707 Phosphorylation
S1836 Phosphorylation
K1866 Methylation
S1917 Phosphorylation
S1930 Phosphorylation
K1934 Methylation
T1935 Phosphorylation
S1936 Phosphorylation
T2068 Phosphorylation
S2070 Phosphorylation
T2083 Phosphorylation
S2086 Phosphorylation
K2185 Ubiquitination
K2196 Ubiquitination
K2203 Methylation
S2270 Phosphorylation
T2283 Phosphorylation
S2288 Phosphorylation
Y2295 Phosphorylation
K2296 Acetylation
S2318 Phosphorylation
S2348 Phosphorylation
S2455 Phosphorylation
S2573 Phosphorylation
Y2604 Phosphorylation
S2605 Phosphorylation
K2691 Ubiquitination
K2700 Ubiquitination

Research Backgrounds

Function:

Histone methyltransferase that methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highest levels in testis. Also found in brain with higher expression in the cerebellum than in any other region, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.

Subunit Structure:

Component of the menin-associated histone methyltransferase complex, at least composed of KMT2B/MLL4, ASH2L, RBBP5, WDR5, DPY30, MEN1; the complex interacts with POLR2A and POLR2B via MEN1. Interacts with NFE2. Interacts with KDM6B. Interacts (via WIN motif) with WDR5. Interacts (via MBM motif) with MEN1.

Family&Domains:

The CXXC zinc finger mediates binding to DNA containing unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides.

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Lysine degradation.

References

1). C-Myc-activated long non-coding RNA LINC01050 promotes gastric cancer growth and metastasis by sponging miR-7161-3p to regulate SPZ1 expression. Journal of Experimental & Clinical Cancer Research, 2021 (PubMed: 34749766) [IF=11.3]

Application: WB    Species: Human    Sample: KATO III cells

Fig. 4 LINC01050 knockdown inhibits gastric cancer cell proliferation and induces apoptosis in vitro and inhibits tumor growth in vivo. a qRT-PCR analysis of LINC01050 expression in BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2. Data are presented as mean ± SD (n = 3). **P

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