CBX8 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from MCF7, using CBX8 Antibody.

Product:	CBX8 Antibody
Catalog:	DF8872
Description:	Rabbit polyclonal antibody to CBX8
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit
Mol.Wt.:	43 kDa; 43kD(Calculated).
Uniprot:	Q9HC52
RRID:	AB_2842068

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Sheep(89%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

CBX8 Antibody detects endogenous levels of total CBX8.

RRID:

AB_2842068
Cite Format: Affinity Biosciences Cat# DF8872, RRID:AB_2842068.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cbx8; CBX8_HUMAN; Chromobox Homolog 8 (Drosophila Pc Class); Chromobox Homolog 8 (Pc Class Homolog, Drosophila); Chromobox Homolog 8; Chromobox protein homolog 8; hPc3; Pc Class 3 Homolog (Drosophila); Pc Class 3 Homolog; Pc3; Polycomb 3; Polycomb 3 homolog; RC1; Rectachrome 1;

Immunogens

Immunogen:

Uniprot:

Q9HC52(CBX8_HUMAN) >>Visit HPA database.

Gene(ID):

CBX8(57332)

Sequence:

Q9HC52 CBX8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPKKRGPKPKTFLLKAQAKAKAKTYEFRSDSARGIRIPYPGRSPQDLASTSRAREGLRNMGLSPPASSTSTSSTCRAEAPRDRDRDRDRDRERDRERERERERERERERERERGTSRVDDKPSSPGDSSKKRGPKPRKELPDPSQRPLGEPSAGLGEYLKGRKLDDTPSGAGKFPAGHSVIQLARRQDSDLVQCGVTSPSSAEATGKLAVDTFPARVIKHRAAFLEAKGQGALDPNGTRVRHGSGPPSSGGGLYRDMGAQGGRPSLIARIPVARILGDPEEESWSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGFFKEKR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Bovine

Sheep

Dog

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HC52 As Substrate

Q9HC52

Site	PTM Type	Source
S4	Phosphorylation	Uniprot
Y28	Phosphorylation	Uniprot
K33	Ubiquitination	Uniprot
K70	Ubiquitination	Uniprot
S110	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
S190	Phosphorylation	Uniprot
S191	Phosphorylation	Uniprot
K205	Ubiquitination	Uniprot
K227	Ubiquitination	Uniprot
T234	Phosphorylation	Uniprot
K240	Acetylation	Uniprot
S256	Phosphorylation	Uniprot
T264	Phosphorylation	Uniprot
S265	Phosphorylation	Uniprot
S268	Phosphorylation	Uniprot
K295	Ubiquitination	Uniprot
T305	Phosphorylation	Uniprot
S311	Phosphorylation	Uniprot
S315	Phosphorylation	Uniprot
Y321	Phosphorylation	Uniprot
S332	Phosphorylation	Uniprot
S352	Phosphorylation	Uniprot
S354	Phosphorylation	Uniprot
K386	Ubiquitination	Uniprot

Research Backgrounds

Q9HC52_CBX8_HUMAN

Function:

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of a PRC1-like complex. Interacts with RING1 RNF2, PCGF1, PCGF2, PCGF3, BMI1, PCGF5 AND PCGF6. Interacts with MLLT3 and histone H3. Interacts with PHC2 (By similarity).

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CBX8 Antibody - #DF8872