Product: SPZ1 Antibody
Catalog: DF8866
Description: Rabbit polyclonal antibody to SPZ1
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Mol.Wt.: 50kD; 49kD(Calculated).
Uniprot: Q9BXG8
RRID: AB_2842063

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
SPZ1 Antibody detects endogenous levels of total SPZ1.
RRID:
AB_2842063
Cite Format: Affinity Biosciences Cat# DF8866, RRID:AB_2842063.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BHLH Zip transcription factor SPZ1; FLJ25709; MGC133987; NYD TSP1; PP1C gamma 2; PP1C gamma; PPP1R148; Protein phosphatase 1, regulatory subunit 148; Spermatogenic leucine zipper 1; Spermatogenic leucine zipper protein 1; Spermatogenic Zip 1; Spz1; Testis secretory sperm-binding protein Li 232m; Testis specific protein 1; Testis specific protein NYD TSP1; TSP1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9BXG8 SPZ1_HUMAN:

Specifically and strongly expressed in the testis. Expressed in several tumor cell lines.

Sequence:
MASSAKSAEMPTISKTVNPTPDPHQEYLDPRITIALFEIGSHSPSSWGSLPFLKNSSHQVTEQQTAQKFNNLLKEIKDILKNMAGFEEKITEAKELFEETNITEDVSAHKENIRGLDKINEMLSTNLPVSLAPEKEDNEKKQEMILETNITEDVSAHKENIRGLDKINEMLSTNLPVSLAPEKEDNEKKQQMIMENQNSENTAQVFARDLVNRLEEKKVLNETQQSQEKAKNRLNVQEETMKIRNNMEQLLQEAEHWSKQHTELSKLIKSYQKSQKDISETLGNNGVGFQTQPNNEVSAKHELEEQVKKLSHDTYSLQLMAALLENECQILQQRVEILKELHHQKQGTLQEKPIQINYKQDKKNQKPSEAKKVEMYKQNKQAMKGTFWKKDRSCRSLDVCLNKKACNTQFNIHVARKALRGKMRSASSLR

PTMs - Q9BXG8 As Substrate

Site PTM Type Enzyme
S265 Phosphorylation
K362 Methylation

Research Backgrounds

Function:

Transcription factor that binds to the DNA sequence 5'-CANNTG-3'(E box) and the G-box motif. May play an important role in the regulation of cell proliferation and differentiation during spermatogenesis (By similarity).

PTMs:

Phosphorylated by MAPK1/ERK2 and MAPK3/ERK1.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically and strongly expressed in the testis. Expressed in several tumor cell lines.

Subunit Structure:

Interacts with PPP1CC isoform gamma-2.

Restrictive clause

 

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