TTF1 Antibody | Affinity Biosciences

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Western blot analysis of TTF1 using HepG2 whole cell lysates.

DF8830 at 1/100 staining Rat lung tissue by IHC-P.

DF8830 at 1/100 staining Human Liver tissue by IHC-P.

Product:	TTF1 Antibody
Catalog:	DF8830
Description:	Rabbit polyclonal antibody to TTF1
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	103 kDa; 103kD(Calculated).
Uniprot:	Q15361
RRID:	AB_2842027

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Bovine(90%), Horse(90%), Sheep(90%), Rabbit(89%), Dog(90%)

Clonality:

Polyclonal

Specificity:

TTF1 Antibody detects endogenous levels of total TTF1.

RRID:

AB_2842027
Cite Format: Affinity Biosciences Cat# DF8830, RRID:AB_2842027.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

RNA polymerase I termination factor; Transcription termination factor 1; Transcription termination factor I; Transcription termination factor RNA polymerase I; TTF 1; TTF-1; TTF-I; Ttf1; TTF1_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q15361(TTF1_HUMAN) >>Visit HPA database.

Gene(ID):

TTF1(7270)

Sequence:

Q15361 TTF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEGESSRFEIHTPVSDKKKKKCSIHKERPQKHSHEIFRDSSLVNEQSQITRRKKRKKDFQHLISSPLKKSRICDETANATSTLKKRKKRRYSALEVDEEAGVTVVLVDKENINNTPKHFRKDVDVVCVDMSIEQKLPRKPKTDKFQVLAKSHAHKSEALHSKVREKKNKKHQRKAASWESQRARDTLPQSESHQEESWLSVGPGGEITELPASAHKNKSKKKKKKSSNREYETLAMPEGSQAGREAGTDMQESQPTVGLDDETPQLLGPTHKKKSKKKKKKKSNHQEFEALAMPEGSQVGSEVGADMQESRPAVGLHGETAGIPAPAYKNKSKKKKKKSNHQEFEAVAMPESLESAYPEGSQVGSEVGTVEGSTALKGFKESNSTKKKSKKRKLTSVKRARVSGDDFSVPSKNSESTLFDSVEGDGAMMEEGVKSRPRQKKTQACLASKHVQEAPRLEPANEEHNVETAEDSEIRYLSADSGDADDSDADLGSAVKQLQEFIPNIKDRATSTIKRMYRDDLERFKEFKAQGVAIKFGKFSVKENKQLEKNVEDFLALTGIESADKLLYTDRYPEEKSVITNLKRRYSFRLHIGRNIARPWKLIYYRAKKMFDVNNYKGRYSEGDTEKLKMYHSLLGNDWKTIGEMVARSSLSVALKFSQISSQRNRGAWSKSETRKLIKAVEEVILKKMSPQELKEVDSKLQENPESCLSIVREKLYKGISWVEVEAKVQTRNWMQCKSKWTEILTKRMTNGRRIYYGMNALRAKVSLIERLYEINVEDTNEIDWEDLASAIGDVPPSYVQTKFSRLKAVYVPFWQKKTFPEIIDYLYETTLPLLKEKLEKMMEKKGTKIQTPAAPKQVFPFRDIFYYEDDSEGEDIEKESEGQAPCMAHACNSSTLGGQGRWII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Dog

Rabbit

Zebrafish

Pig

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15361 As Substrate

Q15361

Site	PTM Type	Source
T12	Phosphorylation	Uniprot
S15	Phosphorylation	Uniprot
S41	Phosphorylation	Uniprot
S47	Phosphorylation	Uniprot
S64	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
S81	Phosphorylation	Uniprot
T82	Phosphorylation	Uniprot
K84	Ubiquitination	Uniprot
K144	Ubiquitination	Uniprot
K150	Ubiquitination	Uniprot
S161	Phosphorylation	Uniprot
S177	Phosphorylation	Uniprot
S213	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
Y231	Phosphorylation	Uniprot
S240	Phosphorylation	Uniprot
T248	Phosphorylation	Uniprot
S253	Phosphorylation	Uniprot
T263	Phosphorylation	Uniprot
S297	Phosphorylation	Uniprot
S301	Phosphorylation	Uniprot
Y357	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
S365	Phosphorylation	Uniprot
K398	Acetylation	Uniprot
S403	Phosphorylation	Uniprot
K434	Ubiquitination	Uniprot
K449	Acetylation	Uniprot
K449	Ubiquitination	Uniprot
S472	Phosphorylation	Uniprot
Y476	Phosphorylation	Uniprot
S478	Phosphorylation	Uniprot
S481	Phosphorylation	Uniprot
S487	Phosphorylation	Uniprot
K506	Ubiquitination	Uniprot
K514	Ubiquitination	Uniprot
K528	Methylation	Uniprot
K528	Ubiquitination	Uniprot
K538	Ubiquitination	Uniprot
K542	Ubiquitination	Uniprot
K549	Ubiquitination	Uniprot
K565	Ubiquitination	Uniprot
K583	Ubiquitination	Uniprot
K617	Ubiquitination	Uniprot
S621	Phosphorylation	Uniprot
K627	Ubiquitination	Uniprot
K629	Ubiquitination	Uniprot
K640	Ubiquitination	Uniprot
S650	Phosphorylation	Uniprot
K671	Ubiquitination	Uniprot
K688	Acetylation	Uniprot
S690	Phosphorylation	Uniprot
K695	Ubiquitination	Uniprot
K700	Acetylation	Uniprot
K700	Ubiquitination	Uniprot
S710	Phosphorylation	Uniprot
K738	Ubiquitination	Uniprot
K740	Ubiquitination	Uniprot
K747	Ubiquitination	Uniprot
K765	Ubiquitination	Uniprot
K808	Ubiquitination	Uniprot
K838	Ubiquitination	Uniprot
K857	Acetylation	Uniprot
K857	Ubiquitination	Uniprot
Y868	Phosphorylation	Uniprot
S872	Phosphorylation	Uniprot

Research Backgrounds

Q15361_TTF1_HUMAN

Function:

Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.

Subunit Structure:

Oligomer. The oligomeric structure enables to interact simultaneously with two separate DNA fragments. Interacts with BAZ2A/TIP5. Interacts with CAVIN1.

Family&Domains:

The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

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TTF1 Antibody - #DF8830