COASY Antibody - #DF8821

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Product: COASY Antibody
Catalog: DF8821
Description: Rabbit polyclonal antibody to COASY
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 62 kDa; 62kD(Calculated).
Uniprot: Q13057
RRID: AB_2842018

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
COASY Antibody detects endogenous levels of total COASY.
RRID:
AB_2842018
Cite Format: Affinity Biosciences Cat# DF8821, RRID:AB_2842018.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bifunctional coenzyme A synthase; Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase; CoA synthase; COASY; COASY_HUMAN; Coenzyme A synthase; Dephospho-CoA kinase; Dephospho-CoA pyrophosphorylase; Dephosphocoenzyme A kinase; DPCK; DPCOAK; NBP; Nucleotide binding protein; Pantetheine-phosphate adenylyltransferase; Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase; pOV 2; POV-2; POV2; PPAT; UKR1;

Immunogens

Immunogen:
Uniprot:

Q13057(COASY_HUMAN) >>Visit HPA database.

Gene(ID):
COASY(80347)
Expression:
Q13057 COASY_HUMAN:

Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.

Sequence:
MAVFRSGLLVLTTPLASLAPRLASILTSAARLVNHTLYVHLQPGMSLEGPAQPQSSPVQATFEVLDFITHLYAGADVHRHLDVRILLTNIRTKSTFLPPLPTSVQNLAHPPEVVLTDFQTLDGSQYNPVKQQLVRYATSCYSCCPRLASVLLYSDYGIGEVPVEPLDVPLPSTIRPASPVAGSPKQPVRGYYRGAVGGTFDRLHNAHKVLLSVACILAQEQLVVGVADKDLLKSKLLPELLQPYTERVEHLSEFLVDIKPSLTFDVIPLLDPYGPAGSDPSLEFLVVSEETYRGGMAINRFRLENDLEELALYQIQLLKDLRHTENEEDKVSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
92
Xenopus
55
Zebrafish
36
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13057 As Substrate

Site PTM Type Enzyme
T13 Phosphorylation
S24 Phosphorylation
S28 Phosphorylation
S172 Phosphorylation
S178 Phosphorylation
S183 Phosphorylation
T199 Phosphorylation
K235 Ubiquitination
K330 Ubiquitination
S369 Phosphorylation
K379 Ubiquitination
K417 Ubiquitination
T441 Phosphorylation
S507 Phosphorylation
K554 Ubiquitination
K558 Ubiquitination

Research Backgrounds

Function:

Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.

Subcellular Location:

Cytoplasm. Mitochondrion matrix.
Note: The protein is mainly present in the mitochondrial matrix, probably anchored to the inner mitochondrial membrane, but is also present in cell lysate.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.

Subunit Structure:

Monomer.

Family&Domains:

In the central section; belongs to the eukaryotic CoaD family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Pantothenate and CoA biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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