PPP2R1A Antibody - #DF8794

(3)  
Product: PPP2R1A Antibody
Catalog: DF8794
Description: Rabbit polyclonal antibody to PPP2R1A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Xenopus
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: P30153
RRID: AB_2841992

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
PPP2R1A Antibody detects endogenous levels of total PPP2R1A.
RRID:
AB_2841992
Cite Format: Affinity Biosciences Cat# DF8794, RRID:AB_2841992.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2AAA_HUMAN; 6330556D22Rik; Alpha isoform of regulatory subunit A, protein phosphatase; DKFZp470L0914; Medium tumor antigen-associated 61 kDa protein; MGC128399; MGC786; MGC83000; MGC95083; PP2A Aalpha; PP2A; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PP2AAALPHA; Ppp2r1a; ppp2r1a-b; PR65; PR65-alpha; PR65A; Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a; Protein phosphatase 2 (formerly 2A), regulatory subunit A,; Protein phosphatase 2 regulatory subunit A alpha; Protein phosphatase 2, 65-KD regulatory subunit A, alpha; Protein phosphatase 2, regulatory subunit A, alpha isoform; Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform;

Immunogens

Immunogen:
Uniprot:

P30153(2AAA_HUMAN) >>Visit HPA database.

Gene(ID):
PPP2R1A(5518)
Sequence:
MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
91
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30153 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S9 Phosphorylation
K34 Ubiquitination
S36 Phosphorylation
T37 Phosphorylation
K107 Ubiquitination
S120 Phosphorylation
S122 Phosphorylation
K133 Ubiquitination
R134 Methylation
K163 Ubiquitination
R167 Methylation
K188 Acetylation
K188 Ubiquitination
K194 Ubiquitination
K202 Ubiquitination
K255 Ubiquitination
K266 Ubiquitination
K272 Ubiquitination
K280 Acetylation
K280 Ubiquitination
K292 Ubiquitination
K305 Ubiquitination
K307 Ubiquitination
S335 Phosphorylation
K342 Ubiquitination
S343 Phosphorylation
S353 Phosphorylation
K374 Ubiquitination
C390 S-Nitrosylation
K416 Ubiquitination
K442 Sumoylation
K467 Ubiquitination
K472 Ubiquitination
K475 Ubiquitination
K485 Ubiquitination
Y495 Phosphorylation
K519 Ubiquitination
R527 Methylation
K542 Ubiquitination
K546 Ubiquitination
S554 Phosphorylation
K561 Acetylation
K561 Ubiquitination
K566 Ubiquitination
T584 Phosphorylation

Research Backgrounds

Function:

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.

Subcellular Location:

Cytoplasm. Nucleus. Chromosome>Centromere. Lateral cell membrane. Cell projection>Dendrite.
Note: Centromeric localization requires the presence of BUB1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity). Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphorylation sites (By similarity). PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGO1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Interacts with CTTNBP2NL. Interacts with GNA12; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT. Interacts with CIP2A; this interaction stabilizes CIP2A. Interacts with FAM122A. Interacts with ADCY8; antagonizes interaction between ADCY8 and calmodulin (By similarity). Interacts with CRTC3 (when phosphorylated at 'Ser-391').

Family&Domains:

Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

Belongs to the phosphatase 2A regulatory subunit A family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

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