ADRA1D Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells(serum starvation treatment), using ADRA1D Antibody.

DF8793 at 1/100 staining Rat brain tissue by IHC-P.

DF8793 at 1/100 staining Rat testis tissue by IHC-P.

Product:	ADRA1D Antibody
Catalog:	DF8793
Description:	Rabbit polyclonal antibody to ADRA1D
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Rabbit
Mol.Wt.:	60 kDa; 60kD(Calculated).
Uniprot:	P25100
RRID:	AB_2841991

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

ADRA1D Antibody detects endogenous levels of total ADRA1D.

RRID:

AB_2841991
Cite Format: Affinity Biosciences Cat# DF8793, RRID:AB_2841991.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADA1D_HUMAN; Adra1; Adra1a; Adra1d; ADRA1R; Adrd1; Adrenergic alpha1A receptor; Adrenergic alpha1D receptor; Adrenergic receptor alpha 1d; Adrenergic receptor delta1; Adrenoceptor alpha 1D; Alpha 1D adrenoceptor; Alpha 1D adrenoreceptor; Alpha adrenergic receptor 1a; Alpha-1A adrenergic receptor; Alpha-1D adrenergic receptor; Alpha-1D adrenoceptor; Alpha-1D adrenoreceptor; Alpha-adrenergic receptor 1a; ALPHA1; Alpha1A adrenergic receptor; Alpha1D adrenergic receptor; Alpha1DAR; DAR; dJ779E11.2; Gpcr8; RA42; Spr8;

Immunogens

Immunogen:

Uniprot:

P25100(ADA1D_HUMAN) >>Visit HPA database.

Gene(ID):

ADRA1D(146)

Sequence:

P25100 ADA1D_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTFRDLLSVSFEGPRPDSSAGGSSAGGGGGSAGGAAPSEGPAVGGVPGGAGGGGGVVGAGSGEDNRSSAGEPGSAGAGGDVNGTAAVGGLVVSAQGVGVGVFLAAFILMAVAGNLLVILSVACNRHLQTVTNYFIVNLAVADLLLSATVLPFSATMEVLGFWAFGRAFCDVWAAVDVLCCTASILSLCTISVDRYVGVRHSLKYPAIMTERKAAAILALLWVVALVVSVGPLLGWKEPVPPDERFCGITEEAGYAVFSSVCSFYLPMAVIVVMYCRVYVVARSTTRSLEAGVKRERGKASEVVLRIHCRGAATGADGAHGMRSAKGHTFRSSLSVRLLKFSREKKAAKTLAIVVGVFVLCWFPFFFVLPLGSLFPQLKPSEGVFKVIFWLGYFNSCVNPLIYPCSSREFKRAFLRLLRCQCRRRRRRRPLWRVYGHHWRASTSGLRQDCAPSSGDAPPGAPLALTALPDPDPEPPGTPEMQAPVASRRKPPSAFREWRLLGPFRRPTTQLRAKVSSLSHKIRAGGAQRAEAACAQRSEVEAVSLGVPHEVAEGATCQAYELADYSNLRETDI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

100

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25100 As Substrate

P25100

Site	PTM Type	Source
S18	Phosphorylation	Uniprot
S201	Phosphorylation	Uniprot
S228	Phosphorylation	Uniprot

Research Backgrounds

P25100_ADA1D_HUMAN

Function:

This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1D sub-subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ADRA1D Antibody - #DF8793