HMG14 Antibody - #DF8777

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Product: HMG14 Antibody
Catalog: DF8777
Description: Rabbit polyclonal antibody to HMG14
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 10 kDa; 11kD(Calculated).
Uniprot: P05114
RRID: AB_2841980

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HMG14 Antibody detects endogenous levels of total HMG14.
RRID:
AB_2841980
Cite Format: Affinity Biosciences Cat# DF8777, RRID:AB_2841980.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ27265; FLJ31471; High mobility group (nonhistone chromosomal) protein 14; High mobility group nucleosome binding 1; High mobility group nucleosome binding domain 1; High mobility group nucleosome binding domain containing protein 1; High mobility group nucleosome-binding domain-containing protein 1; High mobility group protein 14; HMG 14; HMG14; HMGN 1; HMGN1; HMGN1_HUMAN; MGC104230; MGC117425; Non-histone chromosomal protein HMG-14; Nonhistone chromosomal protein HMG 14; Nonhistone chromosomal protein HMG14;

Immunogens

Immunogen:
Uniprot:

P05114(HMGN1_HUMAN) >>Visit HPA database.

Gene(ID):
HMGN1(3150)
Sequence:
MPKRKVSSAEGAAKEEPKRRSARLSAKPPAKVEAKPKKAAAKDKSSDKKVQTKGKRGAKGKQAEVANQETKEDLPAENGETKTEESPASDEAGEKEAKSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05114 As Substrate

Site PTM Type Enzyme
K3 Acetylation
K5 Acetylation
K5 Methylation
K5 Ubiquitination
S7 Phosphorylation P68400 (CSNK2A1) , O75676 (RPS6KA4) , P17612 (PRKACA) , P51812 (RPS6KA3) , O75582 (RPS6KA5) , P17252 (PRKCA)
S8 Phosphorylation P68400 (CSNK2A1)
K14 Acetylation
K14 Ubiquitination
K18 Acetylation
S21 Phosphorylation P51812 (RPS6KA3) , P17612 (PRKACA) , P17252 (PRKCA)
S25 Phosphorylation P17252 (PRKCA) , P51812 (RPS6KA3) , P17612 (PRKACA)
K27 Acetylation
K27 Ubiquitination
K31 Acetylation
K31 Ubiquitination
K38 Acetylation
K42 Acetylation
K48 Acetylation
K53 Acetylation
K55 Acetylation
K59 Acetylation
K61 Acetylation
K61 Ubiquitination
T70 Phosphorylation
K71 Ubiquitination
T81 Phosphorylation
K82 Acetylation
K82 Ubiquitination
T83 Phosphorylation
S86 Phosphorylation P68400 (CSNK2A1)
S89 Phosphorylation P68400 (CSNK2A1)
K95 Ubiquitination
K98 Ubiquitination
S99 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).

PTMs:

Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the HMGN family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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