Product: SAP30 Antibody
Catalog: DF8763
Description: Rabbit polyclonal antibody to SAP30
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: O75446
RRID: AB_2841967

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SAP30 Antibody detects endogenous levels of total SAP30.
RRID:
AB_2841967
Cite Format: Affinity Biosciences Cat# DF8763, RRID:AB_2841967.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

30 kDa Sin3-associated polypeptide; Histone deacetylase complex subunit SAP 30; Histone deacetylase complex subunit SAP30; MSin3A associated polypeptide p30; SAP 30; Sap30; SAP30_HUMAN; Sin3 associated polypeptide 30 kDa; Sin3 associated polypeptide 30kD; Sin3 associated polypeptide; Sin3 corepressor complex subunit SAP 30; Sin3 corepressor complex subunit SAP30; Sin3-associated polypeptide p30; Sin3A associated protein 30kDa; Sin3A associated protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O75446 SAP30_HUMAN:

Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.

Sequence:
MNGFTPDEMSRGGDAAAAVAAVVAAAAAAASAGNGTGAGTGAEVPGAGAVSAAGPPGAAGPGPGQLCCLREDGERCGRAAGNASFSKRIQKSISQKKVKIELDKSARHLYICDYHKNLIQSVRNRRKRKGSDDDGGDSPVQDIDTPEVDLYQLQVNTLRRYKRHFKLPTRPGLNKAQLVEIVGCHFRSIPVNEKDTLTYFIYSVKNDKNKSDLKVDSGVH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75446 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
K87 Sumoylation
K87 Ubiquitination
K91 Ubiquitination
K104 Acetylation
K104 Ubiquitination
S131 Phosphorylation
S138 Phosphorylation
T145 Phosphorylation
Y151 Phosphorylation
K166 Acetylation
T169 Phosphorylation
K175 Acetylation
K175 Ubiquitination
K194 Ubiquitination
T196 Phosphorylation
Y199 Phosphorylation
Y202 Phosphorylation
K205 Sumoylation
K214 Sumoylation
K214 Ubiquitination

Research Backgrounds

Function:

Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.

Subunit Structure:

Interacts with SIN3A, SIN3B, HDAC2 and NCOR1. Interacts with SAMS1. Component of the histone deacetylase complex that includes at least SIN3A, HDAC1 and HDAC2 (By similarity). Interacts with HDAC1. Interacts with HCFC1. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (By similarity).

Family&Domains:

Belongs to the SAP30 family.

Restrictive clause

 

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For Research Use Only.
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