MT-ND3 Antibody - #DF8747

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Product: MT-ND3 Antibody
Catalog: DF8747
Description: Rabbit polyclonal antibody to MT-ND3
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 13 kDa; 13kD(Calculated).
Uniprot: P03897
RRID: AB_2841951

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
MT-ND3 Antibody detects endogenous levels of total MT-ND3.
RRID:
AB_2841951
Cite Format: Affinity Biosciences Cat# DF8747, RRID:AB_2841951.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC=1.6.5.3; Mitochondrially encoded NADH dehydrogenase 3; MT-ND3; MTND3; NADH dehydrogenase subunit 3; NADH dehydrogenase, subunit 3 (complex I); NADH-ubiquinone oxidoreductase chain 3; ND3; NU3M_HUMAN;

Immunogens

Immunogen:
Uniprot:

P03897(NU3M_HUMAN) >>Visit HPA database.

Gene(ID):
MT-ND3(4537)
Sequence:
MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIALLLPLPWALQTTNLPLMVMSSLLLIIILALSLAYEWLQKGLDWTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Zebrafish
89
Chicken
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P03897 As Substrate

Site PTM Type Enzyme
S52 Phosphorylation
Y104 Phosphorylation

Research Backgrounds

Function:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

Subcellular Location:

Mitochondrion membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the complex I subunit 3 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

References

1). Mitochondrial miR-181a-5p promotes glucose metabolism reprogramming in liver cancer by regulating the electron transport chain. CARCINOGENESIS, 2020 (PubMed: 31628462) [IF=4.7]

Application: WB    Species: mouse    Sample: HCC and adjacent normal tissues

Figure 1. |Mitochondrial genome and mitomiR expression profile in HCC and adjacent normal tissues. (A) Heatmap displaying mitochondrial genome transcripts. (B) qRT-PCR analysis of mitochondrially transcribed mRNAs. 12S rRNA served as the internal control to normalize the data. *P < 0.05 was considered statistically significant. (C) Western blotting of mt-ND1, mt-ND5, mt-ND6, mt-CYB, mt-CO2 and mt-ATP8. VADC1/Porin served as the internal control. *P < 0.05 was considered statistically significant.

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