ASAP1 antibody Antibody - #DF8746

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Product: ASAP1 antibody Antibody
Catalog: DF8746
Description: Rabbit polyclonal antibody to ASAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 125kDa; 125kD(Calculated).
Uniprot: Q9ULH1
RRID: AB_2841950

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
ASAP1 antibody Antibody detects endogenous levels of total ASAP1 antibody.
RRID:
AB_2841950
Cite Format: Affinity Biosciences Cat# DF8746, RRID:AB_2841950.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein; 130 kDa phosphatidylinositol 4; 5-biphosphate-dependent ARF1 GTPase-activating protein; ADP ribosylation factor directed GTPase activating protein 1; ADP-ribosylation factor-directed GTPase-activating protein 1; AMAP 1; AMAP1; ANK repeat and PH domain-containing protein 1; ARF GTPase activating protein 1; ARF GTPase-activating protein 1; Arf-GAP with SH3 domain; ASAP 1; ASAP1; ASAP1_HUMAN; Centaurin beta 4; CentB4; DDEF 1; DDEF1 protein; DEF-1; Development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; KIAA1249; PAG 2; PAG2; PAP; PIP2 dependent ARF1 GAP; PIP2-dependent ARF1 GAP; ZG 14P; ZG14P;

Immunogens

Immunogen:
Uniprot:

Q9ULH1(ASAP1_HUMAN) >>Visit HPA database.

Gene(ID):
ASAP1(50807)
Sequence:
MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSTSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVDIVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEIDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNAGKGPTGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDGGPSSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKTDHLSLDKATIPPEIFQKSSQLAELPQKPPPGDLPPKPTELAPKPQIGDLPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGDVSPKAQQPSEVTLKSHPLDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

PTMs - Q9ULH1 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
T34 Phosphorylation
K101 Ubiquitination
S129 Phosphorylation
K182 Acetylation
K184 Acetylation
K209 Ubiquitination
K232 Ubiquitination
K256 Ubiquitination
K276 Ubiquitination
K284 Ubiquitination
S305 Phosphorylation
Y312 Phosphorylation
T372 Phosphorylation
S433 Phosphorylation
S493 Phosphorylation
S514 Phosphorylation
S525 Phosphorylation
K555 Acetylation
K563 Acetylation
S717 Phosphorylation
S726 Phosphorylation
S733 Phosphorylation
S738 Phosphorylation
S744 Phosphorylation
S746 Phosphorylation
S757 Phosphorylation
T758 Phosphorylation
Y767 Phosphorylation Q14289 (PTK2B)
K800 Ubiquitination
S816 Phosphorylation
K824 Ubiquitination
T837 Phosphorylation
S839 Phosphorylation
S843 Phosphorylation
S865 Phosphorylation
S877 Phosphorylation
S881 Phosphorylation
K905 Acetylation
S910 Phosphorylation
T915 Phosphorylation
S924 Phosphorylation
S925 Phosphorylation
S1008 Phosphorylation
S1027 Phosphorylation
S1032 Phosphorylation
S1041 Phosphorylation
S1044 Phosphorylation
T1048 Phosphorylation
T1054 Phosphorylation
T1097 Phosphorylation
S1122 Phosphorylation
S1128 Phosphorylation

Research Backgrounds

Function:

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.

PTMs:

Phosphorylated on tyrosine residues by SRC.

Subcellular Location:

Cytoplasm. Membrane.
Note: Predominantly cytoplasmic. Partially membrane-associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2 (By similarity). Interacts with CTTN. Interacts (via SH3 domain) with APC.

Family&Domains:

The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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