Phospho-WEE1 (Ser642) Antibody - #DF8745

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Product: Phospho-WEE1 (Ser642) Antibody
Catalog: DF8745
Description: Rabbit polyclonal antibody to Phospho-WEE1 (Ser642)
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Rabbit, Dog
Mol.Wt.: 55kD(isoform), 72~100kD; 72kD(Calculated).
Uniprot: P30291
RRID: AB_2841949

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-WEE1 (Ser642) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser642.
RRID:
AB_2841949
Cite Format: Affinity Biosciences Cat# DF8745, RRID:AB_2841949.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686I18166; EC 2.7.10.2; FLJ16446; MGC105683; OTTHUMP00000231338; OTTHUMP00000231339; Wee 1; WEE 1 homolog 1 (S. pombe); WEE1; WEE1 homolog (S. pombe); Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+ homolog; Wee1+ S. pombe homolog; WEE1, S. pombe, homolog of; WEE1, somatic; Wee1-like protein kinase; WEE1_HUMAN; WEE1A; Wee1A kinase; WEE1hu;

Immunogens

Immunogen:
Uniprot:

P30291(WEE1_HUMAN) >>Visit HPA database.

Gene(ID):
WEE1(7465)
Sequence:
MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30291 As Substrate

Site PTM Type Enzyme
Phosphorylation
R14 Methylation
S48 Phosphorylation
T49 Phosphorylation
S53 Phosphorylation P53350 (PLK1)
S67 Phosphorylation
T69 Phosphorylation
S78 Phosphorylation
S85 Phosphorylation
S121 Phosphorylation P68400 (CSNK2A1)
S123 Phosphorylation P53350 (PLK1) , P06493 (CDK1) , P24941 (CDK2)
S127 Phosphorylation
Y132 Phosphorylation
S137 Phosphorylation
S139 Phosphorylation
S150 Phosphorylation
S165 Phosphorylation
T173 Phosphorylation
T187 Phosphorylation
T190 Phosphorylation
S193 Phosphorylation
S196 Phosphorylation
R199 Methylation
K207 Ubiquitination
S211 Phosphorylation P49841 (GSK3B)
S212 Phosphorylation P48730 (CSNK1D)
T217 Phosphorylation
T239 Phosphorylation
S242 Phosphorylation
Y258 Phosphorylation
S262 Phosphorylation
S270 Phosphorylation
Y272 Phosphorylation
K292 Ubiquitination
S293 Phosphorylation
K304 Ubiquitination
S307 Phosphorylation
S312 Phosphorylation
K315 Ubiquitination
S444 Phosphorylation
K460 Ubiquitination
S472 Phosphorylation
S480 Phosphorylation
K547 Ubiquitination
S571 Phosphorylation
S576 Phosphorylation
K588 Ubiquitination
K590 Sumoylation
K590 Ubiquitination
K596 Ubiquitination
K600 Ubiquitination
R617 Methylation
S642 Phosphorylation Q8IWQ3 (BRSK2) , Q8TDC3 (BRSK1) , P31749 (AKT1)
Y646 Phosphorylation

PTMs - P30291 As Enzyme

Substrate Site Source
P06493-2 (CDK1) Y15 Uniprot
P07900 (HSP90AA1) Y38 Uniprot
P24941 (CDK2) T14 Uniprot
P24941-1 (CDK2) Y15 Uniprot
P30291-1 (WEE1) T173 Uniprot

Research Backgrounds

Function:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

PTMs:

Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.

Dephosphorylated at Thr-239 by CTDP1.

Ubiquitinated and degraded at the onset of G2/M phase.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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