Phospho-ICK (Tyr159) Antibody - #DF8739
| Product: | Phospho-ICK (Tyr159) Antibody |
| Catalog: | DF8739 |
| Description: | Rabbit polyclonal antibody to Phospho-ICK (Tyr159) |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 71 kDa; 71kD(Calculated). |
| Uniprot: | Q9UPZ9 |
| RRID: | AB_2841943 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8739, RRID:AB_2841943.
Fold/Unfold
2210420N10Rik; AI848300; ECO; Heart serine threonine protein kinase; hICK; Intestinal cell (MAK like) kinase; Intestinal cell kinase; KIAA0936; Laryngeal cancer kinase 2; LCK2; MAK related kinase; MGC46090; mICK; MRK; OTTMUSP00000041856; OTTMUSP00000041857; Serine/threonine protein kinase ICK;
Immunogens
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.
- Q9UPZ9 CILK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANVVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSKPPYTDYVSTRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTASQALRYPYFQVGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRISSRQHQASQPPLHLTYPYKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSKITAGLEHKNGEIKPKSRRRWGLISRSTKDSDDWADLDDLDFSPSLSRIDLKNKKRQSDDTLCRFESVLDLKPSEPVGTGNSAPTQTSYQRRDTPTLRSAAKQHYLKHSRYLPGISIRNGILSNPGKEFIPPNPWSSSGLSGKSSGTMSVISKVNSVGSSSTSSSGLTGNYVPSFLKKEIGSAMQRVHLAPIPDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRTDWASKYASRR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UPZ9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | P50613 (CDK7) , Q8IZL9 (CDK20) | Uniprot |
| Y159 | Phosphorylation | Q9UPZ9 (ICK) | Uniprot |
| Y177 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| K491 | Methylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| K562 | Acetylation | Uniprot | |
| Y583 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot |
PTMs - Q9UPZ9 As Enzyme
Research Backgrounds
Required for ciliogenesis. Phosphorylates KIF3A (By similarity). Involved in the control of ciliary length. Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (By similarity). May play a key role in the development of multiple organ systems and particularly in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity (By similarity).
Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-157 increases kinase activity.
Nucleus. Cytoplasm>Cytosol. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Also found at the ciliary tip (PubMed:24797473). Nuclear localization has been observed with a GFP-tagged construct in transfected HeLa cells (PubMed:12103360, PubMed:19185282).
Cytoplasm.
Note: Predominant cytoplasmic localization has been observed with a N-terminally GFP-tagged construct.
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
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