Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody - #DF8737

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Product: Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody
Catalog: DF8737
Description: Rabbit polyclonal antibody to Phospho-Ephrin B1/B2/B3 (Tyr324)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
Mol.Wt.: 38 kDa; 38kD,37kD,36kD(Calculated).
Uniprot: P98172 | P52799 | Q15768
RRID: AB_2841941

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324.
RRID:
AB_2841941
Cite Format: Affinity Biosciences Cat# DF8737, RRID:AB_2841941.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CFND; CFNS; Craniofrontonasal syndrome (craniofrontonasal dysplasia); EFL 3; EFL-3; EFL3; EFNB1; EFNB1_HUMAN; Elk L; ELK ligand; ELK-L; Eph related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 2; Ephrin-B1; EPLG2; LERK 2; LERK-2; LERK2; Ligand of eph related kinase 2; MGC8782; EFN B2; EFNB 2; Efnb2; EFNB2_HUMAN; Eph related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 5; ephrin B2; Ephrin-B2; EphrinB2; EPLG 5; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK 5; LERK-5; LERK5; Ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; OTTMUSP00000024973; EFL 6; EFL6; EFNB 3; Efnb3; EFNB3_HUMAN; EPH related receptor transmembrane ligand ELK L3; EPH related receptor transmembrane ligand ELKL3; EPH related receptor tyrosine kinase ligand 8; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 8; Ephrin B3; Ephrin-B3; EphrinB3; EPLG 8; EPLG8; LERK 8; LERK-8; LERK8;

Immunogens

Immunogen:
Uniprot:

P98172(EFNB1_HUMAN) >>Visit HPA database.

P52799(EFNB2_HUMAN) >>Visit HPA database.

Q15768(EFNB3_HUMAN) >>Visit HPA database.

Gene(ID):
EFNB2(1948) | EFNB1(1947) | EFNB3(1949)
Expression:
P98172 EFNB1_HUMAN:

Widely expressed (PubMed:8070404, PubMed:7973638). Detected in both neuronal and non-neuronal tissues (PubMed:8070404, PubMed:7973638). Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord (PubMed:7973638).

P52799 EFNB2_HUMAN:

Lung and kidney.

Q15768 EFNB3_HUMAN:

Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.

Sequence:
MARPGQRWLGKWLVAMVVWALCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDGFFNSKVALFAAVGAGCVIFLLIIIFLTVLLLKLRKRHRKHTQQRAAALSLSTLASPKGGSGTAGTEPSDIIIPLRTTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

MAVRRDSVWKYCWGVLMVLCRTAISKSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGCIIFIVIIITLVVLLLKYRRRHRKHSPQHTTTLSLSTLATPKRSGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

MGPPHSGPGGVRVGALLLLGVLGLVSGLSLEPVYWNSANKRFQAEGGYVLYPQIGDRLDLLCPRARPPGPHSSPNYEFYKLYLVGGAQGRRCEAPPAPNLLLTCDRPDLDLRFTIKFQEYSPNLWGHEFRSHHDYYIIATSDGTREGLESLQGGVCLTRGMKVLLRVGQSPRGGAVPRKPVSEMPMERDRGAAHSLEPGKENLPGDPTSNATSRGAEGPLPPPSMPAVAGAAGGLALLLLGVAGAGGAMCWRRRRAKPSESRHPGPGSFGRGGSLGLGGGGGMGPREAEPGELGIALRGGGAADPPFCPHYEKVSGDYGHPVYIVQDGPPQSPPNIYYKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P98172/P52799/Q15768 As Substrate

Site PTM Type Enzyme
Y11 Phosphorylation
N36 N-Glycosylation
T69 Phosphorylation
T180 O-Glycosylation
T189 O-Glycosylation
S260 Phosphorylation
T264 Phosphorylation
S268 Phosphorylation
S270 Phosphorylation
T274 Phosphorylation
Y304 Phosphorylation P29323 (EPHB2)
S308 Phosphorylation
Y311 Phosphorylation
Y316 Phosphorylation
S325 Phosphorylation
Y330 Phosphorylation
Y331 Phosphorylation
K332 Ubiquitination
Site PTM Type Enzyme
Y79 Phosphorylation
K163 Ubiquitination
T172 O-Glycosylation
T177 O-Glycosylation
T178 O-Glycosylation
S179 O-Glycosylation
T188 O-Glycosylation
S201 O-Glycosylation
T211 O-Glycosylation
S218 Phosphorylation
S223 Phosphorylation
S226 Phosphorylation
S227 Phosphorylation
S281 Phosphorylation
S283 Phosphorylation
T284 Phosphorylation
S287 Phosphorylation
K289 Ubiquitination
Y313 Phosphorylation
Y317 Phosphorylation
K319 Ubiquitination
S321 Phosphorylation
Y324 Phosphorylation
Y329 Phosphorylation
S338 Phosphorylation
Y343 Phosphorylation
Y344 Phosphorylation
K345 Ubiquitination
Site PTM Type Enzyme
S268 Phosphorylation
S274 Phosphorylation
Y311 Phosphorylation
Y318 Phosphorylation
Y323 Phosphorylation
S332 Phosphorylation
Y337 Phosphorylation
Y338 Phosphorylation

Research Backgrounds

Function:

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells. Shows high affinity for the receptor tyrosine kinase EPHB1/ELK. Can also bind EPHB2 and EPHB3. Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

PTMs:

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

Proteolytically processed. The ectodomain is cleaved, probably by a metalloprotease, to produce a membrane-tethered C-terminal fragment. This fragment is then further processed by the gamma-secretase complex to yield a soluble intracellular domain peptide which can translocate to the nucleus. The intracellular domain peptide is highly labile suggesting that it is targeted for degradation by the proteasome.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Membrane raft.
Note: May recruit GRIP1 and GRIP2 to membrane raft domains.

Cell membrane>Single-pass type I membrane protein.

Nucleus.
Note: Colocalizes with ZHX2 in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Detected in both neuronal and non-neuronal tissues. Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord.

Subunit Structure:

Interacts (via PDZ-binding motif) with GRIP1 and GRIP2 (via PDZ domain 6). Interacts with TLE1. The intracellular domain peptide interacts with ZHX2; the interaction enhances ZHX2 transcriptional repression activity (By similarity).

Family&Domains:

Belongs to the ephrin family.

Function:

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.

(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.

PTMs:

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell junction>Adherens junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Lung and kidney.

Subunit Structure:

Interacts with PDZRN3 (By similarity). Binds to the receptor tyrosine kinases EPHA4, EPHB4 and EPHA3.

(Microbial infection) Interacts with Hendra virus and Nipah virus G protein.

Family&Domains:

Belongs to the ephrin family.

Function:

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

(Microbial infection) Acts as a receptor for nipah virus and hendra virus.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.

Subunit Structure:

Interacts with GRIP1 and GRIP2.

(Microbial infection) Interacts with nipah virus and hendra virus glycoprotein.

Family&Domains:

Belongs to the ephrin family.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

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