TPH2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Myeloma cells, using TPH2 Antibody.

DF8732 at 1/100 staining Mouse brain tissue by IHC-P.

Product:	TPH2 Antibody
Catalog:	DF8732
Description:	Rabbit polyclonal antibody to TPH2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	56 kDa; 56kD(Calculated).
Uniprot:	Q8IWU9
RRID:	AB_2841936

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(86%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TPH2 Antibody detects endogenous levels of total TPH2.

RRID:

AB_2841936
Cite Format: Affinity Biosciences Cat# DF8732, RRID:AB_2841936.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC138871; ADHD7; FLJ37295; MGC138872; Neuronal tryptophan hydroxylase; NTPH; TPH 2; Tph2; TPH2_HUMAN; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2; Tryptophan hydroxylase 2;

Immunogens

Immunogen:

Uniprot:

Q8IWU9(TPH2_HUMAN) >>Visit HPA database.

Gene(ID):

TPH2(121278)

Expression:

Q8IWU9 TPH2_HUMAN:

Brain specific.

Sequence:

Q8IWU9 TPH2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQPAMMMFSSKYWARRGFSLDSAVPEEHQLLGSSTLNKPNSGKNDDKGNKGSSKREAATESGKTAVVFSLKNEVGGLVKALRLFQEKRVNMVHIESRKSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPENIWTEEEELEDVPWFPRKISELDKCSHRVLMYGSELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Horse

Bovine

Sheep

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IWU9 As Substrate

Q8IWU9

Site	PTM Type	Enzyme	Source
S19	Phosphorylation	P17612 (PRKACA)	Uniprot
K79	Acetylation		Uniprot
S104	Phosphorylation		Uniprot
Y249	Phosphorylation		Uniprot
Y252	Phosphorylation		Uniprot
S382	Phosphorylation		Uniprot
S392	Phosphorylation		Uniprot

Research Backgrounds

Q8IWU9_TPH2_HUMAN

Tissue Specificity:

Brain specific.

Family&Domains:

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Research Fields

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Serotonergic synapse.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TPH2 Antibody - #DF8732