RELT Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat brain, using RELT Antibody at 1/1000 dilution.

DF8712 at 1/100 staining rat testis tissue by IHC-P.

Product:	RELT Antibody
Catalog:	DF8712
Description:	Rabbit polyclonal antibody to RELT
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Rat, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	46 kDa; 46kD(Calculated).
Uniprot:	Q969Z4
RRID:	AB_2841916

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Rat(%), Bovine(100%), Sheep(100%), Rabbit(90%), Dog(100%)

Clonality:

Polyclonal

Specificity:

RELT Antibody detects endogenous levels of total RELT.

RRID:

AB_2841916
Cite Format: Affinity Biosciences Cat# DF8712, RRID:AB_2841916.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Receptor expressed in lymphoid tissues; Relt; TNFRSF19L; TR19L_HUMAN; Tumor necrosis factor receptor superfamily member 19L;

Immunogens

Immunogen:

Uniprot:

Q969Z4(TR19L_HUMAN) >>Visit HPA database.

Gene(ID):

RELT(84957)

Expression:

Q969Z4 TR19L_HUMAN:

Spleen, lymph node, brain, breast and peripheral blood leukocytes (at protein level) (PubMed:28688764). Expressed highly in bone marrow and fetal liver. Very low levels in skeletal muscle, testis and colon. Not detected in kidney and pancreas.

Sequence:

Q969Z4 TR19L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKPSLLCRPLSCFLMLLPWPLATLTSTTLWQCPPGEEPDLDPGQGTLCRPCPPGTFSAAWGSSPCQPHARCSLWRRLEAQVGMATRDTLCGDCWPGWFGPWGVPRVPCQPCSWAPLGTHGCDEWGRRARRGVEVAAGASSGGETRQPGNGTRAGGPEETAAQYAVIAIVPVFCLMGLLGILVCNLLKRKGYHCTAHKEVGPGPGGGGSGINPAYRTEDANEDTIGVLVRLITEKKENAAALEELLKEYHSKQLVQTSHRPVSKLPPAPPNVPHICPHRHHLHTVQGLASLSGPCCSRCSQKKWPEVLLSPEAVAATTPVPSLLPNPTRVPKAGAKAGRQGEITILSVGRFRVARIPEQRTSSMVSEVKTITEAGPSWGDLPDSPQPGLPPEQQALLGSGGSRTKWLKPPAENKAEENRYVVRLSESNLVI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Rabbit

Pig

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969Z4 As Substrate

Q969Z4

Site	PTM Type	Source
S140	Phosphorylation	Uniprot
Y214	Phosphorylation	Uniprot
T223	Phosphorylation	Uniprot
K235	Ubiquitination	Uniprot
K246	Ubiquitination	Uniprot
K251	Ubiquitination	Uniprot
S299	Phosphorylation	Uniprot
S309	Phosphorylation	Uniprot
T317	Phosphorylation	Uniprot
S321	Phosphorylation	Uniprot
T327	Phosphorylation	Uniprot
K404	Ubiquitination	Uniprot

Research Backgrounds

Q969Z4_TR19L_HUMAN

Function:

May play a role in apoptosis. Induces activation of MAPK14/p38 and MAPK8/JNK MAPK cascades, when overexpressed. Involved in dental enamel formation.

PTMs:

Phosphorylated in vitro by OXSR1. Phosphorylated by STK39.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cytoplasm. Cytoplasm>Perinuclear region.

Tissue Specificity:

Spleen, lymph node, brain, breast and peripheral blood leukocytes (at protein level). Expressed highly in bone marrow and fetal liver. Very low levels in skeletal muscle, testis and colon. Not detected in kidney and pancreas.

Subunit Structure:

Interacts with TRAF1. Interacts with RELL1, RELL2 and OXSR1. Interacts with PLSCR1. Interacts with STK39.

Family&Domains:

Belongs to the RELT family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

Restrictive clause

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RELT Antibody - #DF8712