MDM1 Antibody | Affinity Biosciences

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Western blot analysis of MDM1 using HeLa whole cell lysates.

Product:	MDM1 Antibody
Catalog:	DF8676
Description:	Rabbit polyclonal antibody to MDM1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig
Mol.Wt.:	80 kDa; 81kD(Calculated).
Uniprot:	Q8TC05
RRID:	AB_2841880

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%)

Clonality:

Polyclonal

Specificity:

MDM1 Antibody detects endogenous levels of total MDM1.

RRID:

AB_2841880
Cite Format: Affinity Biosciences Cat# DF8676, RRID:AB_2841880.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ95264; MDM 1; Mdm1; Mdm1 nuclear protein homolog (mouse); Mdm1 nuclear protein homolog; MDM1_HUMAN; Mdm4 transformed 3T3 cell double minute 1 p53 binding protein (mouse); Mdm4 transformed 3T3 cell double minute 1 p53 binding protein; Mouse Mdm1 nuclear protein homolog; Nuclear protein double minute 1; Nuclear protein MDM1;

Immunogens

Immunogen:

Uniprot:

Q8TC05(MDM1_HUMAN) >>Visit HPA database.

Gene(ID):

MDM1(56890)

Sequence:

Q8TC05 MDM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPVRFKGLSEYQRNFLWKKSYLSESCNSSVGRKYPWAGLRSDQLGITKEPSFISKRRVPYHDPQISKSLEWNGAISESNVVASPEPEAPETPKSQEAEQKDVTQERVHSLEASRVPKRTRSHSADSRAEGASDVENNEGVTNHTPVNENVELEHSTKVLSENVDNGLDRLLRKKAGLTVVPSYNALRNSEYQRQFVWKTSKETAPAFAANQVFHNKSQFVPPFKGNSVIHETEYKRNFKGLSPVKEPKLRNDLRENRNLETVSPERKSNKIDDRLKLEAEMELKDLHQPKRKLTPWKHQRLGKVNSEYRAKFLSPAQYLYKAGAWTHVKGNMPNQVKELREKAEFYRKRVQGTHFSRDHLNQILSDSNCCWDVSSTTSSEGTVSSNIRALDLAGDPTSHKTLQKCPSTEPEEKGNIVEEQPQKNTTEKLGVSAPTIPVRRRLAWDTENTSEDVQKQPGEKEEEDDNEEEGDRKTGKQAFMGEQEKLDVREKSKADKMKEGSDSSVSSEKGGRLPTPKLRELGGIQRTHHDLTTPAVGGAVLVSPSKMKPPAPEQRKRMTSQDCLETSKNDFTKKESRAVSLLTSPAAGIKTVDPLPLREDSEDNIHKFAEATLPVSKIPKYPTNPPGQLPSPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTLARAKKRKENFWGKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Xenopus

Dog

Rabbit

Sheep

Horse

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TC05 As Substrate

Q8TC05

Site	PTM Type	Source
K6	Methylation	Uniprot
S29	Phosphorylation	Uniprot
K48	Ubiquitination	Uniprot
S51	Phosphorylation	Uniprot
S83	Phosphorylation	Uniprot
S109	Phosphorylation	Uniprot
S132	Phosphorylation	Uniprot
Y234	Phosphorylation	Uniprot
S242	Phosphorylation	Uniprot
S263	Phosphorylation	Uniprot
T294	Phosphorylation	Uniprot
K297	Ubiquitination	Uniprot
S314	Phosphorylation	Uniprot
S356	Phosphorylation	Uniprot
T408	Phosphorylation	Uniprot
S503	Phosphorylation	Uniprot
S504	Phosphorylation	Uniprot
S506	Phosphorylation	Uniprot
T515	Phosphorylation	Uniprot
S543	Phosphorylation	Uniprot
T572	Phosphorylation	Uniprot
K573	Methylation	Uniprot
K574	Methylation	Uniprot
S584	Phosphorylation	Uniprot
K590	Ubiquitination	Uniprot
S601	Phosphorylation	Uniprot
S631	Phosphorylation	Uniprot
S648	Phosphorylation	Uniprot
S683	Phosphorylation	Uniprot
S686	Phosphorylation	Uniprot

Research Backgrounds

Q8TC05_MDM1_HUMAN

Function:

Microtubule-binding protein that negatively regulates centriole duplication. Binds to and stabilizes microtubules.

Subcellular Location:

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Localizes to the centriole lumen.

Family&Domains:

Belongs to the MDM1 family.

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MDM1 Antibody - #DF8676