MAK Antibody - #DF8675
| Product: | MAK Antibody |
| Catalog: | DF8675 |
| Description: | Rabbit polyclonal antibody to MAK |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 70 kDa; 71kD(Calculated). |
| Uniprot: | P20794 |
| RRID: | AB_2841879 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8675, RRID:AB_2841879.
Fold/Unfold
intestinal cell kinase; dJ417M14.2; fj04c02; Ick; Mak; MAK_HUMAN; Male germ cell associated kinase; Male germ cell-associated kinase; male germ cell-associated kinase retinal-enriched isoform; OTTHUMP00000016025; Rck; RP62; Serine/threonine protein kinase MAK; Serine/threonine-protein kinase MAK; testicular secretory protein Li 28; wu:fj04c02; zgc:56603;
Immunogens
Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
- P20794 MAK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNRYTTMRQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHANVIKLKEVIRENDHLYFIFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHGFFHRDMKPENLLCMGPELVKIADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSVYSSPIDVWAVGSIMAELYMLRPLFPGTSEVDEIFKICQVLGTPKKSDWPEGYQLASSMNFRFPQCVPINLKTLIPNASNEAIQLMTEMLNWDPKKRPTASQALKHPYFQVGQVLGPSSNHLESKQSLNKQLQPLESKPSLVEVEPKPLPDIIDQVVGQPQPKTSQQPLQPIQPPQNLSVQQPPKQQSQEKPPQTLFPSIVKNMPTKPNGTLSHKSGRRRWGQTIFKSGDSWEELEDYDFGASHSKKPSMGVFKEKRKKDSPFRLPEPVPSGSNHSTGENKSLPAVTSLKSDSELSTAPTSKQYYLKQSRYLPGVNPKKVSLIASGKEINPHTWSNQLFPKSLGPVGAELAFKRSNAGNLGSYATYNQSGYIPSFLKKEVQSAGQRIHLAPLNATASEYTWNTKTGRGQFSGRTYNPTAKNLNIVNRAQPIPSVHGRTDWVAKYGGHR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P20794 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y156 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | P20794 (MAK) | Uniprot |
| Y159 | Phosphorylation | P20794 (MAK) | Uniprot |
| S161 | Phosphorylation | Uniprot | |
| T162 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| Y480 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot |
Research Backgrounds
Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
Autophosphorylated. Phosphorylated on serine and threonine residues.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Midbody. Cell projection>Cilium>Photoreceptor outer segment. Photoreceptor inner segment.
Note: Localized in both the connecting cilia and the outer segment axonemes (By similarity). Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase.
Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
Interacts with RP1 (By similarity). Interacts with AR and CDK20. Found in a complex containing MAK, AR and NCOA3. Interacts with FZR1 (via WD repeats).
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
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