Product: IP6K2 Antibody
Catalog: DF8669
Description: Rabbit polyclonal antibody to IP6K2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 49 kDa; 49kD(Calculated).
Uniprot: Q9UHH9
RRID: AB_2841873

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
IP6K2 Antibody detects endogenous levels of total IP6K2.
RRID:
AB_2841873
Cite Format: Affinity Biosciences Cat# DF8669, RRID:AB_2841873.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP 1D myo inositol hexakisphosphate phosphotransferase; IHPK 2; IHPK2; Inositol hexakisphosphate kinase 2; InsP6 kinase 2; Ip6k2; IP6K2_HUMAN; KIAA0263; OTTHUMP00000164824; P(i) uptake stimulator; P(i)-uptake stimulator; Pi uptake stimulator; PiUS;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSPAFRAMDVEPRAKGVLLEPFVHQVGGHSCVLRFNETTLCKPLVPREHQFYETLPAEMRKFTPQYKGVVSVRFEEDEDRNLCLIAYPLKGDHGIVDIVDNSDCEPKSKLLRWTTNKKHHVLETEKTPKDWVRQHRKEEKMKSHKLEEEFEWLKKSEVLYYTVEKKGNISSQLKHYNPWSMKCHQQQLQRMKENAKHRNQYKFILLENLTSRYEVPCVLDLKMGTRQHGDDASEEKAANQIRKCQQSTSAVIGVRVCGMQVYQAGSGQLMFMNKYHGRKLSVQGFKEALFQFFHNGRYLRRELLGPVLKKLTELKAVLERQESYRFYSSSLLVIYDGKERPEVVLDSDAEDLEDLSEESADESAGAYAYKPIGASSVDVRMIDFAHTTCRLYGEDTVVHEGQDAGYIFGLQSLIDIVTEISEESGE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHH9 As Substrate

Site PTM Type Enzyme
K15 Ubiquitination
K42 Ubiquitination
K61 Ubiquitination
K67 Ubiquitination
Y87 Phosphorylation
K90 Ubiquitination
K118 Ubiquitination
K126 Ubiquitination
K129 Ubiquitination
K145 Ubiquitination
K154 Ubiquitination
K155 Ubiquitination
Y160 Phosphorylation
K165 Ubiquitination
K166 Sumoylation
K166 Ubiquitination
K174 Ubiquitination
K182 Ubiquitination
K196 Ubiquitination
K222 Ubiquitination
K236 Ubiquitination
K243 Ubiquitination
K279 Ubiquitination
K315 Ubiquitination
S347 Phosphorylation P68400 (CSNK2A1)
S356 Phosphorylation P68400 (CSNK2A1)
S359 Phosphorylation

Research Backgrounds

Function:

Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the inositol phosphokinase (IPK) family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

References

1). Postnatal Foxp2 regulates early psychiatric-like phenotypes and associated molecular alterations in the R6/1 transgenic mouse model of Huntington's disease. Neurobiology of Disease, 2022 (PubMed: 36029989) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Fig. 4. Molecular and microstructural analysis of the 4-week-old R6/1-Foxp2 mice striatum. (a) Volcano plot of downregulated (red) and upregulated proteins (blue) of R6/1-GFP striatum respect to WT-GFP mice. (b) Volcano plot of downregulated (red) and upregulated proteins (blue) of R6/1-Foxp2 mice striatum respect to WT-GFP mice. (c) Heat map of clusters of downregulated (red) and upregulated proteins (blue) of R6/1-GFP and R6/1-Foxp2 mice striatum respect to WT-GFP mice. Cutoffs of ±0.5-fold change and p < 0.05. (n = 4 mice / group). (d) Golgi-Cox-stained dendrites from medium spiny neurons in the dorsal striatum. Scale bar, 7 μm. (e) Quantification of spine density in dendrites as in d. Two-way ANOVA, group effect: F(1,302) = 9.678; p = 0.0020; treatment effect: F(1,302) = 9.728; p = 0.0020; interaction effect: F(1,302) = 14.65; p = 0.0002. Sidak's post hoc test (15 dendrites per animal / 4–5 mice / group). Densitometric quantification of striatal Ip6k2 (f), Arhgap4 (g) and Tor1A (h) protein levels in WT-GFP, R6/1-GFP, WT-Foxp2 and R6/1-Foxp2 mice. As a loading control α-tubulin was used in f-h. Data are means ± SEM. WT-GFP (n = 7), R6/1 GFP (n = 7); WT Foxp2 (n = 7); R6/1 Foxp2 (n = 7). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

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