HCN2 Antibody - #DF8663
| Product: | HCN2 Antibody |
| Catalog: | DF8663 |
| Description: | Rabbit polyclonal antibody to HCN2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Dog, Chicken |
| Mol.Wt.: | 96 kDa; 97kD(Calculated). |
| Uniprot: | Q9UL51 |
| RRID: | AB_2841867 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8663, RRID:AB_2841867.
Fold/Unfold
BCNG-2; BCNG2; Brain cyclic nucleotide gated channel 2; Brain cyclic nucleotide-gated channel 2; HAC1; Hcn2; HCN2_HUMAN; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2;
Immunogens
- Q9UL51 HCN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGGPRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGPAEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UL51 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S81 | Phosphorylation | Uniprot | |
| T87 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y476 | Phosphorylation | P12931 (SRC) | Uniprot |
| T576 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Q13237 (PRKG2) | Uniprot |
| Y697 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S771 | Phosphorylation | Uniprot | |
| S779 | Phosphorylation | Uniprot | |
| S786 | Phosphorylation | Uniprot | |
| T792 | Phosphorylation | Uniprot | |
| Y795 | Phosphorylation | Uniprot | |
| T851 | Phosphorylation | Uniprot | |
| S860 | Phosphorylation | Uniprot | |
| S868 | Phosphorylation | Uniprot |
Research Backgrounds
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).
Phosphorylation at Ser-668 by PRKG2 shifts the voltage-dependence to more negative voltages, hence counteracting the stimulatory effect of cGMP on gating.
Cell membrane>Multi-pass membrane protein.
Highly expressed throughout the brain. Detected at low levels in heart.
The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits. Heterotetramer with HCN1. Forms an obligate 4:4 complex with accessory subunit PEX5L. Interacts with KCNE2 (By similarity). Homotetramer.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Belongs to the potassium channel HCN family.
Research Fields
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.