GLCTK Antibody | Affinity Biosciences

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Western blot analysis GLCTK using HT29 whole cell lysates.

Product:	GLCTK Antibody
Catalog:	DF8661
Description:	Rabbit polyclonal antibody to GLCTK
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	55 kDa; 55kD(Calculated).
Uniprot:	Q8IVS8
RRID:	AB_2841865

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(91%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

GLCTK Antibody detects endogenous levels of total GLCTK.

RRID:

AB_2841865
Cite Format: Affinity Biosciences Cat# DF8661, RRID:AB_2841865.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CG9886 like; GLCTK_HUMAN; Glycerate kinase; Glyctk; GLYCTK1; HBeAg binding protein 2; HBeAg binding protein 4; HBeAg-binding protein 4; HBeAgBP4A; HBEBP2; HBEBP4;

Immunogens

Immunogen:

Uniprot:

Q8IVS8(GLCTK_HUMAN) >>Visit HPA database.

Gene(ID):

GLYCTK(132158)

Expression:

Q8IVS8 GLCTK_HUMAN:

Widely expressed.

Sequence:

Q8IVS8 GLCTK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKVRDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQTLTRLLAARGATIQELNTIRKALSQLKGGGLAQAAYPAQVVSLILSDVVGDPVEVIASGPTVASSHNVQDCLHILNRYGLRAALPRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVLSAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQIPDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAELRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATFLAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Horse

Zebrafish

Dog

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IVS8 As Substrate

Q8IVS8

Site	PTM Type	Source
S24	Phosphorylation	Uniprot
S30	Phosphorylation	Uniprot
S31	Phosphorylation	Uniprot
S44	Phosphorylation	Uniprot
S60	Phosphorylation	Uniprot
Y274	Phosphorylation	Uniprot
K285	Ubiquitination	Uniprot
S343	Phosphorylation	Uniprot
Y348	Phosphorylation	Uniprot

Research Backgrounds

Q8IVS8_GLCTK_HUMAN

Subcellular Location:

Cytoplasm.

Cytoplasm. Mitochondrion.

Tissue Specificity:

Widely expressed.

Family&Domains:

Belongs to the glycerate kinase type-2 family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GLCTK Antibody - #DF8661