GIT1 Antibody - #DF8660

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Product: GIT1 Antibody
Catalog: DF8660
Description: Rabbit polyclonal antibody to GIT1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 84 kDa; 84kD(Calculated).
Uniprot: Q9Y2X7
RRID: AB_2841864

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
GIT1 Antibody detects endogenous levels of total GIT1.
RRID:
AB_2841864
Cite Format: Affinity Biosciences Cat# DF8660, RRID:AB_2841864.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARF GAP GIT1; ARF GTPase activating protein GIT1; ARF GTPase-activating protein GIT1; CAT 1; CAT-1; CaT1; Cool associated and tyrosine phosphorylated protein 1; Cool-associated and tyrosine-phosphorylated protein 1; G protein coupled receptor kinase interacting ArfGAP 1; G protein coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GIT1_HUMAN; GRK interacting protein 1; GRK-interacting protein 1;

Immunogens

Immunogen:
Uniprot:

Q9Y2X7(GIT1_HUMAN) >>Visit HPA database.

Gene(ID):
GIT1(28964)
Sequence:
MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSLRSQSDLDDQHDYDSVASDEDTDQEPLRSTGATRSNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
88
Chicken
82
Zebrafish
73
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2X7 As Substrate

Site PTM Type Enzyme
S36 Phosphorylation
S46 Phosphorylation O94806 (PRKD3)
K49 Ubiquitination
S54 Phosphorylation
K103 Ubiquitination
Y111 Phosphorylation
S134 Phosphorylation
K135 Ubiquitination
S154 Phosphorylation
T168 Phosphorylation
T169 Phosphorylation
T202 Phosphorylation
Y224 Phosphorylation
Y246 Phosphorylation
S254 Phosphorylation
Y284 Phosphorylation
Y321 Phosphorylation Q05397 (PTK2) , P12931 (SRC)
K358 Ubiquitination
S359 Phosphorylation
S361 Phosphorylation
S362 Phosphorylation
T364 Phosphorylation
S370 Phosphorylation
S373 Phosphorylation
S375 Phosphorylation
Y383 Phosphorylation
S385 Phosphorylation
S388 Phosphorylation
T392 Phosphorylation
S410 Phosphorylation
S413 Phosphorylation
S414 Phosphorylation
S417 Phosphorylation
S448 Phosphorylation
S449 Phosphorylation
T480 Phosphorylation
T491 Phosphorylation
S498 Phosphorylation
T499 Phosphorylation
S508 Phosphorylation Q13153 (PAK1)
Y510 Phosphorylation
S514 Phosphorylation
K517 Methylation
S536 Phosphorylation
T537 Phosphorylation
Y545 Phosphorylation
S546 Phosphorylation
Y554 Phosphorylation
S561 Phosphorylation
T568 Phosphorylation
S570 Phosphorylation
S571 Phosphorylation
S575 Phosphorylation
S577 Phosphorylation
S585 Phosphorylation
S592 Phosphorylation
S596 Phosphorylation
Y598 Phosphorylation
T601 Phosphorylation
K613 Ubiquitination
S630 Phosphorylation
S670 Phosphorylation
C674 S-Nitrosylation
K677 Acetylation
S700 Phosphorylation Q13153 (PAK1)
T753 Phosphorylation

Research Backgrounds

Function:

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).

PTMs:

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).

Subcellular Location:

Cytoplasm.
Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4. Interacts with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1 (By similarity). Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1. Interacts with SCRIB. Interacts (via N- and C-terminus) with ENTR1 (via N-terminus); this interaction is direct. Found in a complex with ENTR1, PTPN13 and GIT1. Interacts with YWHAZ (By similarity).

Family&Domains:

The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

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