ZO-2 Antibody | Affinity Biosciences

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Product:	ZO-2 Antibody
Catalog:	DF8632
Description:	Rabbit polyclonal antibody to ZO-2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	133 kDa; 134kD(Calculated).
Uniprot:	Q9UDY2
RRID:	AB_2841836

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(83%), Dog(92%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

ZO-2 Antibody detects endogenous levels of total ZO-2.

RRID:

AB_2841836
Cite Format: Affinity Biosciences Cat# DF8632, RRID:AB_2841836.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C9DUPq21.11; DFNA51; DUP9q21.11; Friedreich ataxia region gene X104 (tight junction protein ZO-2); MGC26306; PFIC4; Tight junction protein 2; Tight junction protein ZO 2; Tight junction protein ZO-2; TJP2; X104; ZO 2; ZO-2; ZO2; ZO2_HUMAN; Zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2;

Immunogens

Immunogen:

Uniprot:

Q9UDY2(ZO2_HUMAN) >>Visit HPA database.

Gene(ID):

TJP2(9414)

Expression:

Q9UDY2 ZO2_HUMAN:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

Sequence:

Q9UDY2 ZO2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREHPHSRSPSPEPRGRPGPIGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIESNRSFSPEERRHQYSDYDYHSSSEKLKERPSSREDTPSRLSRMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKAAPRTFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGEMVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGNWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRDNAGDRADFWRMRGQRSGVKKNLRKSREDLTAVVSVSTKFPAYERVLLREAGFKRPVVLFGPIADIAMEKLANELPDWFQTAKTEPKDAGSEKSTGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLFDQANKLKKTCAHLFTATINLNSANDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASSDQLRDNSPPPAFKPEPPKAKTQNKEESYDFSKSYEYKSNPSAVAGNETPGASTKGYPPPVAAKPTFGRSILKPSTPIPPQEGEEVGESSEEQDNAPKSVLGKVKIFEKMDHKARLQRMQELQEAQNARIEIAQKHPDIYAVPIKTHKPDPGTPQHTSSRPPEPQKAPSRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Horse

Dog

Chicken

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UDY2 As Substrate

Q9UDY2

Site	PTM Type	Source
S16	Phosphorylation	Uniprot
T91	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
S153	Phosphorylation	Uniprot
S156	Phosphorylation	Uniprot
S163	Phosphorylation	Uniprot
S168	Phosphorylation	Uniprot
S170	Phosphorylation	Uniprot
S174	Phosphorylation	Uniprot
S186	Phosphorylation	Uniprot
S192	Phosphorylation	Uniprot
S196	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
T212	Phosphorylation	Uniprot
S216	Phosphorylation	Uniprot
S220	Phosphorylation	Uniprot
S232	Phosphorylation	Uniprot
S240	Phosphorylation	Uniprot
S244	Phosphorylation	Uniprot
Y249	Phosphorylation	Uniprot
Y253	Phosphorylation	Uniprot
Y257	Phosphorylation	Uniprot
Y261	Phosphorylation	Uniprot
Y265	Phosphorylation	Uniprot
S266	Phosphorylation	Uniprot
Y269	Phosphorylation	Uniprot
S279	Phosphorylation	Uniprot
S284	Phosphorylation	Uniprot
S286	Phosphorylation	Uniprot
S292	Phosphorylation	Uniprot
S294	Phosphorylation	Uniprot
S296	Phosphorylation	Uniprot
Y319	Phosphorylation	Uniprot
S325	Phosphorylation	Uniprot
T333	Phosphorylation	Uniprot
T335	Phosphorylation	Uniprot
T339	Phosphorylation	Uniprot
S385	Phosphorylation	Uniprot
S394	Phosphorylation	Uniprot
S398	Phosphorylation	Uniprot
S400	Phosphorylation	Uniprot
S406	Phosphorylation	Uniprot
S413	Phosphorylation	Uniprot
S415	Phosphorylation	Uniprot
Y423	Phosphorylation	Uniprot
S424	Phosphorylation	Uniprot
Y426	Phosphorylation	Uniprot
Y428	Phosphorylation	Uniprot
S430	Phosphorylation	Uniprot
S431	Phosphorylation	Uniprot
S432	Phosphorylation	Uniprot
S440	Phosphorylation	Uniprot
S441	Phosphorylation	Uniprot
T445	Phosphorylation	Uniprot
S450	Phosphorylation	Uniprot
T455	Phosphorylation	Uniprot
T457	Phosphorylation	Uniprot
K460	Methylation	Uniprot
S461	Phosphorylation	Uniprot
T462	Phosphorylation	Uniprot
T468	Phosphorylation	Uniprot
K475	Ubiquitination	Uniprot
Y479	Phosphorylation	Uniprot
T494	Phosphorylation	Uniprot
S499	Phosphorylation	Uniprot
Y506	Phosphorylation	Uniprot
T510	Phosphorylation	Uniprot
S520	Phosphorylation	Uniprot
Y574	Phosphorylation	Uniprot
T585	Phosphorylation	Uniprot
S590	Phosphorylation	Uniprot
S606	Phosphorylation	Uniprot
Y638	Phosphorylation	Uniprot
S702	Phosphorylation	Uniprot
T707	Phosphorylation	Uniprot
S711	Phosphorylation	Uniprot
S713	Phosphorylation	Uniprot
K730	Methylation	Uniprot
T760	Phosphorylation	Uniprot
S767	Phosphorylation	Uniprot
K769	Ubiquitination	Uniprot
S770	Phosphorylation	Uniprot
T771	Phosphorylation	Uniprot
T778	Phosphorylation	Uniprot
T831	Phosphorylation	Uniprot
S832	Phosphorylation	Uniprot
S836	Phosphorylation	Uniprot
S886	Phosphorylation	Uniprot
S902	Phosphorylation	Uniprot
Y903	Phosphorylation	Uniprot
T905	Phosphorylation	Uniprot
Y911	Phosphorylation	Uniprot
S913	Phosphorylation	Uniprot
S916	Phosphorylation	Uniprot
S920	Phosphorylation	Uniprot
T925	Phosphorylation	Uniprot
Y932	Phosphorylation	Uniprot
T933	Phosphorylation	Uniprot
S953	Phosphorylation	Uniprot
S961	Phosphorylation	Uniprot
S966	Phosphorylation	Uniprot
S978	Phosphorylation	Uniprot
S979	Phosphorylation	Uniprot
S986	Phosphorylation	Uniprot
S1006	Phosphorylation	Uniprot
Y1007	Phosphorylation	Uniprot
S1012	Phosphorylation	Uniprot
Y1013	Phosphorylation	Uniprot
Y1015	Phosphorylation	Uniprot
K1016	Ubiquitination	Uniprot
T1027	Phosphorylation	Uniprot
S1031	Phosphorylation	Uniprot
S1048	Phosphorylation	Uniprot
S1053	Phosphorylation	Uniprot
T1054	Phosphorylation	Uniprot
S1067	Phosphorylation	Uniprot
S1068	Phosphorylation	Uniprot
Y1118	Phosphorylation	Uniprot
T1124	Phosphorylation	Uniprot
T1131	Phosphorylation	Uniprot
T1135	Phosphorylation	Uniprot
S1137	Phosphorylation	Uniprot
S1147	Phosphorylation	Uniprot
Y1150	Phosphorylation	Uniprot
S1156	Phosphorylation	Uniprot
Y1157	Phosphorylation	Uniprot
S1159	Phosphorylation	Uniprot
Y1166	Phosphorylation	Uniprot
S1171	Phosphorylation	Uniprot
S1174	Phosphorylation	Uniprot
R1176	Methylation	Uniprot
Y1178	Phosphorylation	Uniprot
Y1179	Phosphorylation	Uniprot
S1182	Phosphorylation	Uniprot

Research Backgrounds

Q9UDY2_ZO2_HUMAN

Function:

Plays a role in tight junctions and adherens junctions.

Subcellular Location:

Cell junction>Adherens junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Nucleus.
Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.

Tissue Specificity:

Subunit Structure:

Homodimer, and heterodimer with ZO1. Interacts with occludin, SAFB and UBN1. Interaction with SAFB occurs in the nucleus. Interacts with SCRIB. Interacts with USP53 (via the C-terminal region) (By similarity).

Family&Domains:

Belongs to the MAGUK family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

References

1). Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. PHARMACOLOGICAL RESEARCH (PubMed: 31863867) [IF=9.3]

Application: WB Species: Mice Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.

2). A novel nano material for anti-cerebral ischemia: Preparation and Application of Borneol Angelica Polysaccharide Liposomes. JOURNAL OF LIPOSOME RESEARCH (PubMed: 35875973) [IF=4.4]

3). Preparation of Borneol Angelica Polysaccharide Liposomes and Study Evaluation of Its Anti-Cerebral Ischemia-Reperfusion Inflammatory Reaction. Research Square

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ZO-2 Antibody - #DF8632