Trypsin-3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/5

Western blot analysis of extracts from various samples, using Trypsin-3 Antibody.

DF8625 at 1/100 staining human pancreatic cancer and para-carcinoma tissue by IHC-P.

DF8625 at 1/100 staining human pancreatic cancer by IHC-P.

DF8625 at 1/100 staining mouse liver tissue by IHC-P.

DF8625 staining A549 cells by IF/ICC.

Product:	Trypsin-3 Antibody
Catalog:	DF8625
Description:	Rabbit polyclonal antibody to Trypsin-3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	32 kDa; 33kD(Calculated).
Uniprot:	P35030
RRID:	AB_2841829

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Trypsin-3 Antibody detects endogenous levels of total Trypsin-3.

RRID:

AB_2841829
Cite Format: Affinity Biosciences Cat# DF8625, RRID:AB_2841829.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Brain trypsinogen; Mesotrypsin; Mesotrypsinogen; MTG; Pancreatic trypsinogen III; Protease, serine, 3; Protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3; TRY3_HUMAN; TRY4; Trypsin 3; Trypsin III; Trypsin IV; Trypsin-3; Trypsinogen 4; Trypsinogen 5; Trypsinogen IV;

Immunogens

Immunogen:

Uniprot:

P35030(TRY3_HUMAN) >>Visit HPA database.

Gene(ID):

PRSS3(5646)

Expression:

P35030 TRY3_HUMAN:

Detected in pancreas and pancreatic fluid (at protein level) (PubMed:6698368). Expressed in pancreas and brain (PubMed:8294000). Detected in ileum (PubMed:12021776).

Sequence:

P35030 TRY3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCGPDDRCPARWPGPGRAVKCGKGLAAARPGRVERGGAQRGGAGLELHPLLGGRTWRAARDADGCEALGTVAVPFDDDDKIVGGYTCEENSLPYQVSLNSGSHFCGGSLISEQWVVSAAHCYKTRIQVRLGEHNIKVLEGNEQFINAAKIIRHPKYNRDTLDNDIMLIKLSSPAVINARVSTISLPTTPPAAGTECLISGWGNTLSFGADYPDELKCLDAPVLTQAECKASYPGKITNSMFCVGFLEGGKDSCQRDSGGPVVCNGQLQGVVSWGHGCAWKNRPGVYTKVYNYVDWIKDTIAANS

PTMs - P35030 As Substrate

P35030

Site	PTM Type	Source
K136	Acetylation	Uniprot
K149	Ubiquitination	Uniprot
K155	Ubiquitination	Uniprot
K169	Ubiquitination	Uniprot
Y232	Phosphorylation	Uniprot

Research Backgrounds

P35030_TRY3_HUMAN

Function:

Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors.

Subcellular Location:

Secreted.

Tissue Specificity:

Detected in pancreas and pancreatic fluid (at protein level). Expressed in pancreas and brain. Detected in ileum.

Family&Domains:

Belongs to the peptidase S1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Protein digestion and absorption.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Trypsin-3 Antibody - #DF8625