Product: Serotonin transporter Antibody
Catalog: DF8616
Description: Rabbit polyclonal antibody to Serotonin transporter
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 70 kDa; 70kD(Calculated).
Uniprot: P31645
RRID: AB_2841820

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Dog(92%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
Serotonin transporter Antibody detects endogenous levels of total Serotonin transporter.
RRID:
AB_2841820
Cite Format: Affinity Biosciences Cat# DF8616, RRID:AB_2841820.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 HTT; 5 HTTLPR; 5 hydroxytryptamine (serotonin) transporter; 5 hydroxytryptamine transporter; 5HT transporter; 5HTT; hSERT; HTT; Na+/Cl- dependent serotonin transporter; OCD1; SC6A4_HUMAN; Serotonin transporter 1; SERT; SERT1; Slc6a4; Sodium dependent serotonin transporter; Sodium-dependent serotonin transporter; Solute carrier family 6 (neurotransmitter transporter) member 4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; Solute carrier family 6 member 4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P31645 SC6A4_HUMAN:

Expressed in platelets (at protein level).

Sequence:
METTPLNSQKQLSACEDGEDCQENGVLQKVVPTPGDKVESGQISNGYSAVPSPGAGDDTRHSIPATTTTLVAELHQGERETWGKKVDFLLSVIGYAVDLGNVWRFPYICYQNGGGAFLLPYTIMAIFGGIPLFYMELALGQYHRNGCISIWRKICPIFKGIGYAICIIAFYIASYYNTIMAWALYYLISSFTDQLPWTSCKNSWNTGNCTNYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGGISWQLALCIMLIFTVIYFSIWKGVKTSGKVVWVTATFPYIILSVLLVRGATLPGAWRGVLFYLKPNWQKLLETGVWIDAAAQIFFSLGPGFGVLLAFASYNKFNNNCYQDALVTSVVNCMTSFVSGFVIFTVLGYMAEMRNEDVSEVAKDAGPSLLFITYAEAIANMPASTFFAIIFFLMLITLGLDSTFAGLEGVITAVLDEFPHVWAKRRERFVLAVVITCFFGSLVTLTFGGAYVVKLLEEYATGPAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIICSFLMSPPQLRLFQYNYPYWSIILGYCIGTSSFICIPTYIAYRLIITPGTFKERIIKSITPETPTEIPCGDIRLNAV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
92
Sheep
92
Dog
92
Chicken
80
Rabbit
75
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31645 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation Q9UQM7 (CAMK2A)
Y47 Phosphorylation
T59 Phosphorylation
S62 Phosphorylation
T81 Phosphorylation
Y142 Phosphorylation
S149 Phosphorylation P17252 (PRKCA)
N208 N-Glycosylation
N217 N-Glycosylation
T276 Phosphorylation Q13976 (PRKG1)
S277 Phosphorylation P17252 (PRKCA)
T603 Phosphorylation P17252 (PRKCA)
S611 Phosphorylation
T613 Phosphorylation
T616 Phosphorylation P28482 (MAPK1) , P45984 (MAPK9) , P27361 (MAPK3) , Q16539 (MAPK14)

Research Backgrounds

Function:

Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

PTMs:

Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.

Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation. Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Endomembrane system>Multi-pass membrane protein. Endosome membrane>Multi-pass membrane protein. Cell junction>Synapse. Cell junction>Focal adhesion.
Note: Could be part of recycling endosomes (PubMed:18227069, PubMed:16870614). Density of transporter molecules on the plasma membrane is itself regulated by STX1A (By similarity). Density of transporter molecules on the plasma membrane is also regulated by serotonin (PubMed:17506858) (PubMed:18227069). Density of transporter molecules seems to be modulated by ITGAV:ITGB3 (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in platelets (at protein level).

Subunit Structure:

Monomer or homooligomer (By similarity). Interacts with TGFB1I1. Interacts (via sialylated form) with MYH9. Interacts with SEC23A, SEC24C and PATJ. Interacts with NOS1; the interaction may diminish the cell surface localization of SERT in the brain and, correspondingly, reduce serotonin reuptake. Interacts with filamentous actin and STX1A (By similarity). Interacts (via C-terminus) with VIM. Interacts (via C-terminus) with SCAMP2; the interaction is direct and retains transporter molecules intracellularly. Interacts with RAB4 (GTP-bound form); the interaction retains transporter molecules intracellularly. Interacts with ITGAV:ITGB3 (By similarity).

Family&Domains:

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Research Fields

· Organismal Systems > Nervous system > Serotonergic synapse.

References

1). Maternal Long-Term Intake of Inulin Improves Fetal Development through Gut Microbiota and Related Metabolites in a Rat Model. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022 (PubMed: 35129337) [IF=6.1]

2). Serotonin transporter and cholecystokinin in diarrhea-predominant irritable bowel syndrome: Associations with abdominal pain, visceral hypersensitivity and psychological performance. World Journal of Clinical Cases, 2020 (PubMed: 32432141) [IF=1.1]

Application: IHC    Species: human    Sample: Mucosal

Figure 1 |Mucosal histology and immunohistochemistry in patients with diarrhea-predominant irritable bowel syndrome. A and B: Mucosal cholecystokininexpression in diarrhea-predominant irritable bowel syndrome patients; C and D: Mucosal serotonin transporter expression in diarrhea-predominant irritable bowelsyndrome patients. CCK: Cholecystokinin; SERT: Serotonin transporter.

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