SEMA4C Antibody | Affinity Biosciences

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Western blot analysis SEMA4C using HeLa whole cell lysates.

Product:	SEMA4C Antibody
Catalog:	DF8612
Description:	Rabbit polyclonal antibody to SEMA4C
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	92 kDa; 93kD(Calculated).
Uniprot:	Q9C0C4
RRID:	AB_2841816

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

SEMA4C Antibody detects endogenous levels of total SEMA4C.

RRID:

AB_2841816
Cite Format: Affinity Biosciences Cat# DF8612, RRID:AB_2841816.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KIAA1739; M-SEMA-F; S4c; SEM4C_HUMAN; Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C; SEMA4C; SEMACL1; SEMAF; SEMAI; Semaphorin-4C; UNQ5855/PRO34487;

Immunogens

Immunogen:

Uniprot:

Q9C0C4(SEM4C_HUMAN) >>Visit HPA database.

Gene(ID):

SEMA4C(54910)

Sequence:

Q9C0C4 SEM4C_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPHWAVWLLAARLWGLGIGAEVWWNLVPRKTVSSGELATVVRRFSQTGIQDFLTLTLTEPTGLLYVGAREALFAFSMEALELQGAISWEAPVEKKTECIQKGKNNQTECFNFIRFLQPYNASHLYVCGTYAFQPKCTYVNMLTFTLEHGEFEDGKGKCPYDPAKGHAGLLVDGELYSATLNNFLGTEPIILRNMGPHHSMKTEYLAFWLNEPHFVGSAYVPESVGSFTGDDDKVYFFFRERAVESDCYAEQVVARVARVCKGDMGGARTLQRKWTTFLKARLACSAPNWQLYFNQLQAMHTLQDTSWHNTTFFGVFQAQWGDMYLSAICEYQLEEIQRVFEGPYKEYHEEAQKWDRYTDPVPSPRPGSCINNWHRRHGYTSSLELPDNILNFVKKHPLMEEQVGPRWSRPLLVKKGTNFTHLVADRVTGLDGATYTVLFIGTGDGWLLKAVSLGPWVHLIEELQLFDQEPMRSLVLSQSKKLLFAGSRSQLVQLPVADCMKYRSCADCVLARDPYCAWSVNTSRCVAVGGHSGSLLIQHVMTSDTSGICNLRGSKKVRPTPKNITVVAGTDLVLPCHLSSNLAHARWTFGGRDLPAEQPGSFLYDARLQALVVMAAQPRHAGAYHCFSEEQGARLAAEGYLVAVVAGPSVTLEARAPLENLGLVWLAVVALGAVCLVLLLLVLSLRRRLREELEKGAKATERTLVYPLELPKEPTSPPFRPCPEPDEKLWDPVGYYYSDGSLKIVPGHARCQPGGGPPSPPPGIPGQPLPSPTRLHLGGGRNSNANGYVRLQLGGEDRGGLGHPLPELADELRRKLQQRQPLPDSNPEESSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Pig

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9C0C4 As Substrate

Q9C0C4

Site	PTM Type	Source
Y249	Phosphorylation	Uniprot
K481	Ubiquitination	Uniprot
S488	Phosphorylation	Uniprot
T704	Phosphorylation	Uniprot
Y707	Phosphorylation	Uniprot
K713	Ubiquitination	Uniprot
S717	Phosphorylation	Uniprot
K729	Ubiquitination	Uniprot
Y736	Phosphorylation	Uniprot
Y737	Phosphorylation	Uniprot
S739	Phosphorylation	Uniprot
S742	Phosphorylation	Uniprot
K744	Ubiquitination	Uniprot
S760	Phosphorylation	Uniprot
S772	Phosphorylation	Uniprot
Y789	Phosphorylation	Uniprot
S832	Phosphorylation	Uniprot

Research Backgrounds

Q9C0C4_SEM4C_HUMAN

Function:

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.

Subcellular Location:

Cell junction>Synapse>Postsynaptic density membrane>Single-pass type I membrane protein. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass type I membrane protein.

Subunit Structure:

Interacts (via the PDZ-binding motif) with GIPC (via the PDZ domain) (By similarity). Interacts with NCDN (By similarity). Interacts (via the PDZ-binding motif) with DLG4. Interacts with PLXNB2 (By similarity).

Family&Domains:

Belongs to the semaphorin family.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

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SEMA4C Antibody - #DF8612