SEMA3A Antibody - #DF8609

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Product: SEMA3A Antibody
Catalog: DF8609
Description: Rabbit polyclonal antibody to SEMA3A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 70~100kDa; 89kD(Calculated).
Uniprot: Q14563
RRID: AB_2841813

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
SEMA3A Antibody detects endogenous levels of total SEMA3A.
RRID:
AB_2841813
Cite Format: Affinity Biosciences Cat# DF8609, RRID:AB_2841813.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Coll 1; Coll1; Collapsin 1; HH16; Hsema I; Hsema III; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Sema III; SEMA1; SEMA1-PEN; SEMA3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A precursor; Semaphorin III; semaphorin L; Semaphorin like growth cone guidance homolog of chick collapsin; Semaphorin-D; Semaphorin3A; SemD;

Immunogens

Immunogen:
Uniprot:

Q14563(SEM3A_HUMAN) >>Visit HPA database.

Gene(ID):
SEMA3A(10371)
Expression:
Q14563 SEM3A_HUMAN:

Expressed in the dorsal root ganglia.

Sequence:
MGWLTRIVCLFWGVLLTARANYQNGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRRTRRQDIRNGDPLTHCSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEERKEEIRVDDHIIRTDQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQKVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFERAPRSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
91
Xenopus
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14563 As Substrate

Site PTM Type Enzyme
S42 Phosphorylation
S405 Phosphorylation
Y410 Phosphorylation
S698 Phosphorylation
K727 Ubiquitination

Research Backgrounds

Function:

Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the dorsal root ganglia.

Subunit Structure:

Interacts with PLXND1.

Family&Domains:

Strong binding to neuropilin is mediated by the carboxy third of the protein.

Belongs to the semaphorin family.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Astragaloside Ⅳ negatively regulates Gpr97-TPL2 signaling to protect against hyperhomocysteine-exacerbated sepsis associated acute kidney injury. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38237511) [IF=7.9]
2). Luteolin Stimulates Proliferation and Inhibits Late Differentiation of Primary Rat Calvarial Osteoblast Induced by High-dose Dexamethasone via Sema3A /NRP1/Pleixin A1. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2021 (PubMed: 33327910) [IF=2.8]

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